The effects of inhibiting P18^INK4C expression on the invasion of gastric adenocarcinoma cell line

Using cDNA microarray with double dots of 4096 human genes, P18 ^INK4C, a member of CKI, was found down-regulated in a gastric adenocarcinoma metastatic cell line (RF-48), compared with the corresponding primary cancer cell line (RF-1), which implied that P18^INK4C might be involved in cell invasion and metastatic progression of human gastric adenocarcinoma. Antisense RNA expression plasmid was applied to inhibit P18 ^INK4C expression to study the effect of decreased P18 ^INK4C expression on cell migration, invasion and proliferation ability and cell cycle of RF-1. Results showed that inhibition of P18 ^INK4C expression could obviously enhance cell invasion ability of RF-1, but had little effect on its cell cycle and cell migration and proliferation ability. These results implied that P18^INK4C might play a pivotal role in regulating cell invasion, rather than regulating cell cycle and proliferation in the progression of human gastric adenocarcinoma as expected before.