The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster

Vladimir Spiegelman; Serge Y. Fuchs; Gennady A. Belitsky

doi:10.1006/bbrc.1997.6273

The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster

Vladimir Spiegelman
, Serge Y. Fuchs
, Gennady A. Belitsky

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd¹) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.

Original language	English (US)
Pages (from-to)	304-307
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	232
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1997.6273
State	Published - Mar 17 1997

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1997.6273

Cite this

@article{0178efe8f03640d6887cdfdebe857ad2,

title = "The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster",

abstract = "The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.",

author = "Vladimir Spiegelman and Fuchs, \{Serge Y.\} and Belitsky, \{Gennady A.\}",

note = "Funding Information: Support by International Science Foundation grant (N1F300 to G.A.B.) is acknowledged. The critical comments of Dr.Zeev Ronai are most appreciated. We thank Dr. Kholodova for 20HE, Dr.W rgler for 91R strain, and Mid-American Drosophila Stock Center for ecd1 strain.",

year = "1997",

month = mar,

day = "17",

doi = "10.1006/bbrc.1997.6273",

language = "English (US)",

volume = "232",

pages = "304--307",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster

AU - Spiegelman, Vladimir

AU - Fuchs, Serge Y.

AU - Belitsky, Gennady A.

N1 - Funding Information: Support by International Science Foundation grant (N1F300 to G.A.B.) is acknowledged. The critical comments of Dr.Zeev Ronai are most appreciated. We thank Dr. Kholodova for 20HE, Dr.W rgler for 91R strain, and Mid-American Drosophila Stock Center for ecd1 strain.

PY - 1997/3/17

Y1 - 1997/3/17

N2 - The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.

AB - The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.

UR - https://www.scopus.com/pages/publications/0031575771

UR - https://www.scopus.com/pages/publications/0031575771#tab=citedBy

U2 - 10.1006/bbrc.1997.6273

DO - 10.1006/bbrc.1997.6273

M3 - Article

C2 - 9125169

AN - SCOPUS:0031575771

SN - 0006-291X

VL - 232

SP - 304

EP - 307

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this