TY - JOUR
T1 - The Genome of Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae)
T2 - Adaptation for Success
AU - Oppert, Brenda
AU - Muszewska, Anna
AU - Steczkiewicz, Kamil
AU - Šatović-Vukšić, Eva
AU - Plohl, Miroslav
AU - Fabrick, Jeffrey A.
AU - Vinokurov, Konstantin S.
AU - Koloniuk, Igor
AU - Johnston, J. Spencer
AU - Smith, Timothy P.L.
AU - Guedes, Raul Narciso C.
AU - Terra, Walter R.
AU - Ferreira, Clélia
AU - Dias, Renata O.
AU - Chaply, Konstantin A.
AU - Elpidina, Elena N.
AU - Tereshchenkova, Valeriia F.
AU - Mitchell, Robert F.
AU - Jenson, Audra J.
AU - McKay, Rachel
AU - Shan, Tisheng
AU - Cao, Xiaolong
AU - Miao, Zelong
AU - Xiong, Chao
AU - Jiang, Haobo
AU - Morrison, William R.
AU - Koren, Sergey
AU - Schlipalius, David
AU - Lorenzen, Marcé D.
AU - Bansal, Raman
AU - Wang, Yu Hui
AU - Perkin, Lindsey
AU - Poelchau, Monica
AU - Friesen, Kenlee
AU - Olmstead, Morgan L.
AU - Scully, Erin
AU - Campbell, James F.
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/3
Y1 - 2022/3
N2 - The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), is a major global pest of cereal grains. Infestations are difficult to control as larvae feed inside grain kernels, and many populations are resistant to both contact insecticides and fumigants. We sequenced the genome of R. dominica to identify genes responsible for important biological functions and develop more targeted and efficacious management strategies. The genome was assembled from long read sequencing and long-range scaffolding technologies. The genome assembly is 479.1 Mb, close to the predicted genome size of 480.4 Mb by flow cytometry. This assembly is among the most contiguous beetle assembly published to date, with 139 scaffolds, an N50 of 53.6 Mb, and L50 of 4, indicating chromosomescale scaffolds. Predicted genes from biologically relevant groups were manually annotated using transcriptome data from adults and different larval tissues to guide annotation. The expansion of carbohydrase and serine peptidase genes suggest that they combine to enable efficient digestion of cereal proteins. A reduction in the copy number of several detoxification gene families relative to other coleopterans may reflect the low selective pressure on these genes in an insect that spends most of its life feeding internally. Chemoreceptor genes contain elevated numbers of pseudogenes for odorant receptors that also may be related to the recent ontogenetic shift of R. dominica to a diet consisting primarily of stored grains. Analysis of repetitive sequences will further define the evolution of bostrichid beetles compared to other species. The data overall contribute significantly to coleopteran genetic research.
AB - The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), is a major global pest of cereal grains. Infestations are difficult to control as larvae feed inside grain kernels, and many populations are resistant to both contact insecticides and fumigants. We sequenced the genome of R. dominica to identify genes responsible for important biological functions and develop more targeted and efficacious management strategies. The genome was assembled from long read sequencing and long-range scaffolding technologies. The genome assembly is 479.1 Mb, close to the predicted genome size of 480.4 Mb by flow cytometry. This assembly is among the most contiguous beetle assembly published to date, with 139 scaffolds, an N50 of 53.6 Mb, and L50 of 4, indicating chromosomescale scaffolds. Predicted genes from biologically relevant groups were manually annotated using transcriptome data from adults and different larval tissues to guide annotation. The expansion of carbohydrase and serine peptidase genes suggest that they combine to enable efficient digestion of cereal proteins. A reduction in the copy number of several detoxification gene families relative to other coleopterans may reflect the low selective pressure on these genes in an insect that spends most of its life feeding internally. Chemoreceptor genes contain elevated numbers of pseudogenes for odorant receptors that also may be related to the recent ontogenetic shift of R. dominica to a diet consisting primarily of stored grains. Analysis of repetitive sequences will further define the evolution of bostrichid beetles compared to other species. The data overall contribute significantly to coleopteran genetic research.
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U2 - 10.3390/genes13030446
DO - 10.3390/genes13030446
M3 - Article
C2 - 35328000
AN - SCOPUS:85126428660
SN - 2073-4425
VL - 13
JO - Genes
JF - Genes
IS - 3
M1 - 446
ER -