The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism

E. Virginia Armbrust; John A. Berges; Chris Bowler; Beverley R. Green; Diego Martinez; Nicholas H. Putnam; Shiguo Zhou; Andrew E. Allen; Kirk E. Apt; Michael Bechner; Mark A. Brzezinski; Balbir K. Chaal; Anthony Chiovitti; Aubrey K. Davis; Mark S. Demarest; J. Chris Detter; Tijana Glavina; David Goodstein; Masood Z. Hadi; Uffe Hellsten; Mark Hildebrand; Bethany D. Jenkins; Jerzy Jurka; Vladimir V. Kapitonov; Nils Kröger; Winnie W.Y. Lau; Todd W. Lane; Frank W. Larimer; J. Casey Lippmeier; Susan Lucas; Mónica Medina; Anton Montsant; Miroslav Obornik; Micaela Schnitzler Parker; Brian Palenik; Gregory J. Pazour; Paul M. Richardson; Tatiana A. Rynearson; Mak A. Saito; David C. Schwartz; Kimberlee Thamatrakoln; Klaus Valentin; Assaf Vardi; Frances P. Wilkerson; Daniel S. Rokhsar

doi:10.1126/science.1101156

The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism

E. Virginia Armbrust, John A. Berges, Chris Bowler, Beverley R. Green, Diego Martinez, Nicholas H. Putnam, Shiguo Zhou, Andrew E. Allen, Kirk E. Apt, Michael Bechner, Mark A. Brzezinski, Balbir K. Chaal, Anthony Chiovitti, Aubrey K. Davis, Mark S. Demarest, J. Chris Detter, Tijana Glavina, David Goodstein, Masood Z. Hadi, Uffe HellstenMark Hildebrand, Bethany D. Jenkins, Jerzy Jurka, Vladimir V. Kapitonov, Nils Kröger, Winnie W.Y. Lau, Todd W. Lane, Frank W. Larimer, J. Casey Lippmeier, Susan Lucas, Mónica Medina, Anton Montsant, Miroslav Obornik, Micaela Schnitzler Parker, Brian Palenik, Gregory J. Pazour, Paul M. Richardson, Tatiana A. Rynearson, Mak A. Saito, David C. Schwartz, Kimberlee Thamatrakoln, Klaus Valentin, Assaf Vardi, Frances P. Wilkerson, Daniel S. Rokhsar

Research output: Contribution to journal › Article › peer-review

1713 Scopus citations

Abstract

Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for ∼20% of global carbon fixation. We report the 34 million - base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand - base pair plastid and 44 thousand - base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.

Original language	English (US)
Pages (from-to)	79-86
Number of pages	8
Journal	Science
Volume	306
Issue number	5693
DOIs	https://doi.org/10.1126/science.1101156
State	Published - Oct 1 2004

All Science Journal Classification (ASJC) codes

General

Access to Document

10.1126/science.1101156

Cite this

Armbrust, E. V., Berges, J. A., Bowler, C., Green, B. R., Martinez, D., Putnam, N. H., Zhou, S., Allen, A. E., Apt, K. E., Bechner, M., Brzezinski, M. A., Chaal, B. K., Chiovitti, A., Davis, A. K., Demarest, M. S., Detter, J. C., Glavina, T., Goodstein, D., Hadi, M. Z., ... Rokhsar, D. S. (2004). The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism. Science, 306(5693), 79-86. https://doi.org/10.1126/science.1101156

@article{57e942af11e946a5a796e04084615843,

title = "The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism",

abstract = "Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for ∼20% of global carbon fixation. We report the 34 million - base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand - base pair plastid and 44 thousand - base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.",

author = "Armbrust, {E. Virginia} and Berges, {John A.} and Chris Bowler and Green, {Beverley R.} and Diego Martinez and Putnam, {Nicholas H.} and Shiguo Zhou and Allen, {Andrew E.} and Apt, {Kirk E.} and Michael Bechner and Brzezinski, {Mark A.} and Chaal, {Balbir K.} and Anthony Chiovitti and Davis, {Aubrey K.} and Demarest, {Mark S.} and Detter, {J. Chris} and Tijana Glavina and David Goodstein and Hadi, {Masood Z.} and Uffe Hellsten and Mark Hildebrand and Jenkins, {Bethany D.} and Jerzy Jurka and Kapitonov, {Vladimir V.} and Nils Kr{\"o}ger and Lau, {Winnie W.Y.} and Lane, {Todd W.} and Larimer, {Frank W.} and Lippmeier, {J. Casey} and Susan Lucas and M{\'o}nica Medina and Anton Montsant and Miroslav Obornik and Parker, {Micaela Schnitzler} and Brian Palenik and Pazour, {Gregory J.} and Richardson, {Paul M.} and Rynearson, {Tatiana A.} and Saito, {Mak A.} and Schwartz, {David C.} and Kimberlee Thamatrakoln and Klaus Valentin and Assaf Vardi and Wilkerson, {Frances P.} and Rokhsar, {Daniel S.}",

year = "2004",

month = oct,

day = "1",

doi = "10.1126/science.1101156",

language = "English (US)",

volume = "306",

pages = "79--86",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5693",

}

Armbrust, EV, Berges, JA, Bowler, C, Green, BR, Martinez, D, Putnam, NH, Zhou, S, Allen, AE, Apt, KE, Bechner, M, Brzezinski, MA, Chaal, BK, Chiovitti, A, Davis, AK, Demarest, MS, Detter, JC, Glavina, T, Goodstein, D, Hadi, MZ, Hellsten, U, Hildebrand, M, Jenkins, BD, Jurka, J, Kapitonov, VV, Kröger, N, Lau, WWY, Lane, TW, Larimer, FW, Lippmeier, JC, Lucas, S, Medina, M, Montsant, A, Obornik, M, Parker, MS, Palenik, B, Pazour, GJ, Richardson, PM, Rynearson, TA, Saito, MA, Schwartz, DC, Thamatrakoln, K, Valentin, K, Vardi, A, Wilkerson, FP & Rokhsar, DS 2004, 'The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism', Science, vol. 306, no. 5693, pp. 79-86. https://doi.org/10.1126/science.1101156

TY - JOUR

T1 - The genome of the diatom Thalassiosira Pseudonana

T2 - Ecology, evolution, and metabolism

AU - Armbrust, E. Virginia

AU - Berges, John A.

AU - Bowler, Chris

AU - Green, Beverley R.

AU - Martinez, Diego

AU - Putnam, Nicholas H.

AU - Zhou, Shiguo

AU - Allen, Andrew E.

AU - Apt, Kirk E.

AU - Bechner, Michael

AU - Brzezinski, Mark A.

AU - Chaal, Balbir K.

AU - Chiovitti, Anthony

AU - Davis, Aubrey K.

AU - Demarest, Mark S.

AU - Detter, J. Chris

AU - Glavina, Tijana

AU - Goodstein, David

AU - Hadi, Masood Z.

AU - Hellsten, Uffe

AU - Hildebrand, Mark

AU - Jenkins, Bethany D.

AU - Jurka, Jerzy

AU - Kapitonov, Vladimir V.

AU - Kröger, Nils

AU - Lau, Winnie W.Y.

AU - Lane, Todd W.

AU - Larimer, Frank W.

AU - Lippmeier, J. Casey

AU - Lucas, Susan

AU - Medina, Mónica

AU - Montsant, Anton

AU - Obornik, Miroslav

AU - Parker, Micaela Schnitzler

AU - Palenik, Brian

AU - Pazour, Gregory J.

AU - Richardson, Paul M.

AU - Rynearson, Tatiana A.

AU - Saito, Mak A.

AU - Schwartz, David C.

AU - Thamatrakoln, Kimberlee

AU - Valentin, Klaus

AU - Vardi, Assaf

AU - Wilkerson, Frances P.

AU - Rokhsar, Daniel S.

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for ∼20% of global carbon fixation. We report the 34 million - base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand - base pair plastid and 44 thousand - base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.

AB - Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for ∼20% of global carbon fixation. We report the 34 million - base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand - base pair plastid and 44 thousand - base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.

UR - http://www.scopus.com/inward/record.url?scp=6044252163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6044252163&partnerID=8YFLogxK

U2 - 10.1126/science.1101156

DO - 10.1126/science.1101156

M3 - Article

C2 - 15459382

AN - SCOPUS:6044252163

SN - 0036-8075

VL - 306

SP - 79

EP - 86

JO - Science

JF - Science

IS - 5693

ER -

The genome of the diatom Thalassiosira Pseudonana: Ecology, evolution, and metabolism

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this