TY - JOUR
T1 - The influence of photosymbiosis in Cassiopea xamachana regenerative success
AU - Mammone, Marta
AU - Sharp, Victoria
AU - Hewitt, Michael
AU - Medina, Mónica
N1 - Funding Information:
We thank the Miyashiro Lab and the Huck Microscopy Facility (Pennsylvania State University) for training and access to confocal microscopy. We also thank the LaJeunesse Lab (Pennsylvania State University) for the supply of algal cultures. MaM thanks the European Marine Research Network for the Individual Fellowship Grant. MM was funded by Penn State internal funds. We also thank Drs. Raúl Gonzalez-Pech, William Fitt, and Dietrich Hofmann for their invaluable feedback on this manuscript. We thank Dr. Uri Frank for his help with our EdU labeling protocol, and Dr. Isaac Wright for continuous patience and help with statistical analysis.
Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/5
Y1 - 2023/5
N2 - The regenerative capacity of Scyphozoans (Phylum Cnidaria) has been relatively understudied. The model organism Cassiopea xamachana hosts photosynthetic dinoflagellate symbionts in the host’s motile amoebocyte cells. A handful of studies have reported regeneration in the polyps of C. xamachana, but the mechanisms underlying regeneration have not been fully explored. Despite undergoing drastic developmental changes when symbiotic, the effect of symbiont presence and species on host regeneration has never been explored. C. xamachana polyps were decapitated when aposymbiotic, and symbiotic with both a homologous and a heterologous symbiont species. Regeneration and asexual budding were observed, and EdU labeling was performed to observe patterns of cell proliferation in regenerating polyps. The presence of symbionts increased likelihood to regenerate, yet symbiont species did not affect success of regeneration. No blastema or dividing cells were observed, implying cell proliferation is not the primary mechanism behind regeneration in polyps of C. xamachana.
AB - The regenerative capacity of Scyphozoans (Phylum Cnidaria) has been relatively understudied. The model organism Cassiopea xamachana hosts photosynthetic dinoflagellate symbionts in the host’s motile amoebocyte cells. A handful of studies have reported regeneration in the polyps of C. xamachana, but the mechanisms underlying regeneration have not been fully explored. Despite undergoing drastic developmental changes when symbiotic, the effect of symbiont presence and species on host regeneration has never been explored. C. xamachana polyps were decapitated when aposymbiotic, and symbiotic with both a homologous and a heterologous symbiont species. Regeneration and asexual budding were observed, and EdU labeling was performed to observe patterns of cell proliferation in regenerating polyps. The presence of symbionts increased likelihood to regenerate, yet symbiont species did not affect success of regeneration. No blastema or dividing cells were observed, implying cell proliferation is not the primary mechanism behind regeneration in polyps of C. xamachana.
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U2 - 10.1007/s13199-023-00920-0
DO - 10.1007/s13199-023-00920-0
M3 - Article
AN - SCOPUS:85156214946
SN - 0334-5114
VL - 90
SP - 61
EP - 70
JO - Symbiosis
JF - Symbiosis
IS - 1
ER -