TY - JOUR
T1 - The inverted repeats of Tn5 are functionally different
AU - Rothstein, Steven J.
AU - Jorgensen, Richard A.
AU - Postle, Kathleen
AU - Reznikoff, William S.
N1 - Funding Information:
We are grateful for support from the NIH to W.S.R.W.S.R. was also supported in part by a Career Development Award from the NIH and was a recipient of the Harry and Evelyn Steenbock Career Development Award.
PY - 1980/3
Y1 - 1980/3
N2 - The inverted repeats of Tn5, which have identical restriction endonuclease cleavage patterns, have different functional properties. They differ with respect to RNA polymerase binding, full promotion of neomycin resistance, the polypeptides coded for by the repeats and their function in the transposition process. There is a weak RNA polymerase binding site present in one repeat and not in the other which seems to be important for neomycin resistance. The two inverted repeats code for polypeptides of different molecular weights, with each repeat appearing to encode two polypeptides. The polypeptides from only one of the repeats of Tn5 appear to be absolutely required for Tn5 transposition.
AB - The inverted repeats of Tn5, which have identical restriction endonuclease cleavage patterns, have different functional properties. They differ with respect to RNA polymerase binding, full promotion of neomycin resistance, the polypeptides coded for by the repeats and their function in the transposition process. There is a weak RNA polymerase binding site present in one repeat and not in the other which seems to be important for neomycin resistance. The two inverted repeats code for polypeptides of different molecular weights, with each repeat appearing to encode two polypeptides. The polypeptides from only one of the repeats of Tn5 appear to be absolutely required for Tn5 transposition.
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U2 - 10.1016/S0092-8674(80)80055-9
DO - 10.1016/S0092-8674(80)80055-9
M3 - Article
C2 - 6244898
AN - SCOPUS:0018864338
SN - 0092-8674
VL - 19
SP - 795
EP - 805
JO - Cell
JF - Cell
IS - 3
ER -