TY - JOUR
T1 - The late region differentially regulates the in Vitro transformation by cottontail rabbit papillomavirus DNA in different cell types
AU - Meyers, Craig
AU - Wettstein, Felix O.
N1 - Funding Information:
We thank David Batey. Hongman Le, and Margerita Tayag for expert technical assistance and Jeannie Martinez and Tina Meyers for help in typing the manuscript . The research was supported by U .S . Public Health Service Grant CA-18151 from the National Cancer Institute and C .M . was a recipient of a predoctoral fellowship from U .S . Public Health Service Grants GM 07104 and Al 07323 .
PY - 1991/4
Y1 - 1991/4
N2 - Papilloma induced by the cottontail rabbit papillomavirus (CRPV) progress at a high frequency to cancers. This property makes the CRPV system unique to study the role of papillomaviruses in cancer development. In contrast to bovine papillomavirus type 1, no convenient in vitro transformation system for CRPV has been available. Here, we describe two in vitro systems that we have developed. Transformation of NIH 3T3 cells is greatly facilitated by deletions in the CRPV late region. Specifically, a 300-bp segment located in the 5′ half of open reading frame L2 inhibits transformation of NIH 3T3 cells by CRPV DNA. In a second system involving the natural host cell of CRPV, rabbit skin epithelial cells, transformation is a two-stage phenomenon leading first to cells with an increased growth potential which subsequently become morphologically transformed. Transformation of rabbit skin epithelial cells does not require deletions in the L2 ORF. However, late region sequences located in the 3' half of ORF L1 are needed, as they are for papilloma induction in rabbits.
AB - Papilloma induced by the cottontail rabbit papillomavirus (CRPV) progress at a high frequency to cancers. This property makes the CRPV system unique to study the role of papillomaviruses in cancer development. In contrast to bovine papillomavirus type 1, no convenient in vitro transformation system for CRPV has been available. Here, we describe two in vitro systems that we have developed. Transformation of NIH 3T3 cells is greatly facilitated by deletions in the CRPV late region. Specifically, a 300-bp segment located in the 5′ half of open reading frame L2 inhibits transformation of NIH 3T3 cells by CRPV DNA. In a second system involving the natural host cell of CRPV, rabbit skin epithelial cells, transformation is a two-stage phenomenon leading first to cells with an increased growth potential which subsequently become morphologically transformed. Transformation of rabbit skin epithelial cells does not require deletions in the L2 ORF. However, late region sequences located in the 3' half of ORF L1 are needed, as they are for papilloma induction in rabbits.
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U2 - 10.1016/0042-6822(91)90897-K
DO - 10.1016/0042-6822(91)90897-K
M3 - Article
C2 - 1849681
AN - SCOPUS:0025857231
SN - 0042-6822
VL - 181
SP - 637
EP - 646
JO - Virology
JF - Virology
IS - 2
ER -