The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

Yu Zhang Cao; Andrea M. Mastro; Mary Lou Eskew; George Hildenbrandt; Padala V. Reddy; C. Channa Reddy

doi:10.1006/bbrc.1995.2857

The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

Yu Zhang Cao, Andrea M. Mastro, Mary Lou Eskew, George Hildenbrandt, Padala V. Reddy, C. Channa Reddy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1-¹⁴C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.

Original language	English (US)
Pages (from-to)	908-915
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	217
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1995.2857
State	Published - Dec 26 1995

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes",

abstract = "Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1-14C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.",

author = "Cao, {Yu Zhang} and Mastro, {Andrea M.} and Eskew, {Mary Lou} and George Hildenbrandt and Reddy, {Padala V.} and Reddy, {C. Channa}",

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T1 - The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

AU - Cao, Yu Zhang

AU - Mastro, Andrea M.

AU - Eskew, Mary Lou

AU - Hildenbrandt, George

AU - Reddy, Padala V.

AU - Reddy, C. Channa

PY - 1995/12/26

Y1 - 1995/12/26

N2 - Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1-14C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.

AB - Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1-14C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.

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The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

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