TY - JOUR
T1 - The morphology of self-assembled lipid-based nanoparticles affects their uptake by cancer cells
AU - Aresh, Wafa
AU - Liu, Ying
AU - Sine, Jessica
AU - Thayer, Derek
AU - Puri, Anu
AU - Huang, Yike
AU - Wang, Yong
AU - Nieh, Mu Ping
N1 - Publisher Copyright:
Copyright © 2016 American Scientific Publishers All rights reserved.
PY - 2016/10
Y1 - 2016/10
N2 - The morphology of nanoparticles (NPs) has been presumed to play an important role in cellular uptake and in vivo stability. This report experimentally demonstrates such dependence by using two types of uniform-sized self-assembled lipid-based NPs, namely nanodiscs and nanovesicles, composed of identical lipid composition. The morphology is characterized by small angle neutron scattering, dynamic light scattering and transmission electron microscopy. Both NPs have similar bio-stability in serum and cellular cytotoxicity. However, cellular uptake of the nanodiscs at 37 °C is consistently and significantly higher than that of the vesicles according to the uptake results of several human cancer cell lines, i.e., CCRFCEM, KB, and OVCAR-8, indicating a strong morphological dependence of cellular internalization. Further studies on such morphological dependence using CCRF-CEM reveals that vesicles only use Clathrin- and caveolae-mediated endocytic pathways, while nanodiscs also take the additional routes of macropinocytosis and microtubule-mediated endocytosis.
AB - The morphology of nanoparticles (NPs) has been presumed to play an important role in cellular uptake and in vivo stability. This report experimentally demonstrates such dependence by using two types of uniform-sized self-assembled lipid-based NPs, namely nanodiscs and nanovesicles, composed of identical lipid composition. The morphology is characterized by small angle neutron scattering, dynamic light scattering and transmission electron microscopy. Both NPs have similar bio-stability in serum and cellular cytotoxicity. However, cellular uptake of the nanodiscs at 37 °C is consistently and significantly higher than that of the vesicles according to the uptake results of several human cancer cell lines, i.e., CCRFCEM, KB, and OVCAR-8, indicating a strong morphological dependence of cellular internalization. Further studies on such morphological dependence using CCRF-CEM reveals that vesicles only use Clathrin- and caveolae-mediated endocytic pathways, while nanodiscs also take the additional routes of macropinocytosis and microtubule-mediated endocytosis.
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U2 - 10.1166/jbn.2016.2292
DO - 10.1166/jbn.2016.2292
M3 - Article
C2 - 29359898
AN - SCOPUS:84990842391
SN - 1550-7033
VL - 12
SP - 1852
EP - 1863
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
IS - 10
ER -