TY - JOUR
T1 - The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway
AU - Chen, Xu
AU - Wang, Yaofeng
AU - Lv, Bo
AU - Li, Jie
AU - Luo, Liqiong
AU - Lu, Songchong
AU - Zhang, Xuan
AU - Ma, Hong
AU - Ming, Feng
N1 - Funding Information:
This work was supported by the Ministry of Agriculture of China [grant No. 2008ZX08009-001-008]; the Natural Science Foundation of Shanghai [grant No. 12ZR1402300].
PY - 2014/3
Y1 - 2014/3
N2 - Plants respond to environmental stresses by altering gene expression, and several genes have been found to mediate stress-induced expression, but many additional factors are yet to be identified. OsNAP is a member of the NAC transcription factor family; it is localized in the nucleus, and shows transcriptional activator activity in yeast. Analysis of the OsNAP transcript levels in rice showed that this gene was significantly induced by ABA and abiotic stresses, including high salinity, drought and low temperature. Rice plants overexpressing OsNAP did not show growth retardation, but showed a significantly reduced rate of water loss, enhanced tolerance to high salinity, drought and low temperature at the vegetative stage, and improved yield under drought stress at the flowering stage. Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59). Our data suggest that OsNAP functions as a transcriptional activator that plays a role in mediating abiotic stress responses in rice.
AB - Plants respond to environmental stresses by altering gene expression, and several genes have been found to mediate stress-induced expression, but many additional factors are yet to be identified. OsNAP is a member of the NAC transcription factor family; it is localized in the nucleus, and shows transcriptional activator activity in yeast. Analysis of the OsNAP transcript levels in rice showed that this gene was significantly induced by ABA and abiotic stresses, including high salinity, drought and low temperature. Rice plants overexpressing OsNAP did not show growth retardation, but showed a significantly reduced rate of water loss, enhanced tolerance to high salinity, drought and low temperature at the vegetative stage, and improved yield under drought stress at the flowering stage. Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59). Our data suggest that OsNAP functions as a transcriptional activator that plays a role in mediating abiotic stress responses in rice.
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U2 - 10.1093/pcp/pct204
DO - 10.1093/pcp/pct204
M3 - Article
C2 - 24399239
AN - SCOPUS:84899066007
SN - 0032-0781
VL - 55
SP - 604
EP - 619
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 3
ER -