The number of accessible SH-groups in Escherichia coli membrane vesicles is increased by ATP or by formate

Nelli Mnatsakanyan, Anna Poladian, Karine Bagramyan, Armen Trchounian

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a ∼1.4-fold increase in the number of accessible SH-groups. The increase was inhibited by treatment with N-ethylmaleimide or the presence of the F0F1-ATPase inhibitors N,N-dicyclohexylcarbodiimide or sodium azide. The increase in accessible SH-groups was also absent in strains with the ATP synthase operon deleted or with the single F0 domain cysteine Cysb21 changed to Ala. Using hyc and hyf mutants, it was shown that the increase was also largely dependent on hydrogenase 4 or hydrogenase 3, main components of formate hydrogen lyase, when bacteria were grown in the absence or presence of added formate. These results suggest a relationship between the F0F1-ATP synthase and hydrogenase 4 or hydrogenase 3 under fermentation conditions.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number3
DOIs
StatePublished - Aug 29 2003

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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