TY - JOUR
T1 - The number of accessible SH-groups in Escherichia coli membrane vesicles is increased by ATP or by formate
AU - Mnatsakanyan, Nelli
AU - Poladian, Anna
AU - Bagramyan, Karine
AU - Trchounian, Armen
N1 - Funding Information:
We thank Dr. S.C. Andrews and Prof. W. Epstein for supplying E. coli strains, valuable advice, and discussion. The study was supported by the Ministry of Education and Science of the Republic of Armenia and by a grant from the United States Civilian Research and Development Foundation (AB1-2307-YE02).
PY - 2003/8/29
Y1 - 2003/8/29
N2 - The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a ∼1.4-fold increase in the number of accessible SH-groups. The increase was inhibited by treatment with N-ethylmaleimide or the presence of the F0F1-ATPase inhibitors N,N′-dicyclohexylcarbodiimide or sodium azide. The increase in accessible SH-groups was also absent in strains with the ATP synthase operon deleted or with the single F0 domain cysteine Cysb21 changed to Ala. Using hyc and hyf mutants, it was shown that the increase was also largely dependent on hydrogenase 4 or hydrogenase 3, main components of formate hydrogen lyase, when bacteria were grown in the absence or presence of added formate. These results suggest a relationship between the F0F1-ATP synthase and hydrogenase 4 or hydrogenase 3 under fermentation conditions.
AB - The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a ∼1.4-fold increase in the number of accessible SH-groups. The increase was inhibited by treatment with N-ethylmaleimide or the presence of the F0F1-ATPase inhibitors N,N′-dicyclohexylcarbodiimide or sodium azide. The increase in accessible SH-groups was also absent in strains with the ATP synthase operon deleted or with the single F0 domain cysteine Cysb21 changed to Ala. Using hyc and hyf mutants, it was shown that the increase was also largely dependent on hydrogenase 4 or hydrogenase 3, main components of formate hydrogen lyase, when bacteria were grown in the absence or presence of added formate. These results suggest a relationship between the F0F1-ATP synthase and hydrogenase 4 or hydrogenase 3 under fermentation conditions.
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U2 - 10.1016/S0006-291X(03)01460-8
DO - 10.1016/S0006-291X(03)01460-8
M3 - Article
C2 - 12914800
AN - SCOPUS:0042665925
SN - 0006-291X
VL - 308
SP - 655
EP - 659
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -