Abstract
To elucidate the complex structure of the human immunoglobulin λ gene locus, a 1020-kb contig was constructed using 184 cosmid clones and one bacterial artificial chromosome (BAC) clone. A high-resolution physical map of this contig revealed that the entire λ gene locus is 911 kb in length. It contains seven constant region (C(λ)) gene segments and 69 unique EcoRI-HindIII segments that hybridize to variable region gene (V(λ) probes. The VpreB gene, BCRL4, and γ-glutamyl transpeptidase gene (GGT)-like sequences are also located within the λ gene locus. Hybridization analysis suggested that the λ gene locus has undergone extensive amplification events in evolution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-135 |
| Number of pages | 11 |
| Journal | Genome research |
| Volume | 5 |
| Issue number | 2 |
| State | Published - 1995 |
All Science Journal Classification (ASJC) codes
- Genetics
- Genetics(clinical)