TY - JOUR
T1 - The ornithine decarboxylase gene of Trypanosoma brucei
T2 - Evidence for horizontal gene transfer from a vertebrate source
AU - Steglich, Carolyn
AU - Schaeffer, Stephen W.
N1 - Funding Information:
Complete genomic sequences were used to determine if the ODC gene was in the same conserved linkage group in all kinetoplasts. Synteny occurs when a gene is on the same chromosome in two or more species. A conserved linkage group occurs when two or more genes are syntenic and are in the same order in multiple species ( Ehrlich et al., 1997 ). All preliminary genomic sequence data for L. major , T. brucei , and T. cruzi were obtained from the GeneDB web site. Genomic sequencing for L. major (strain MHOM/1L/81/Freidlin) was accomplished as part of the Leishmania Genome Network; version 4 (July 2004) was used for this analysis. Genomic sequencing for T. brucei (stock TREU927/4 single VAT derivative GuTat10.1) was accomplished as part of the Trypanosoma Genome Network; version 3 (July 2004) was used. Most of the genomic data for these two species were provided by the Wellcome Trust Sanger Institute and The Institute for Genomic Research (TIGR), which are supported by the Wellcome Trust and the National Institutes of Health, USA (grant AI043062), respectively. Both of these genomes have been manually annotated in the versions used. The sequencing centers involved in the Trypanosoma cruzi (strain CL Brener) genome project are: TIGR, the Karolinska Institute and the Seattle Biomedical Research Institute, funded by the National Institute of Allergy and Infectious Disease (NIAID). Version 3 (July, 2004) of this genomic sequence was used; gene annotations are automated ones only.
PY - 2006/5
Y1 - 2006/5
N2 - Kinetoplastid protozoans in the family Trypanosomatidae are parasites, many of them responsible for serious diseases in humans and domestic animals. Ornithine decarboxlyase (ODC), a protein at the core of polyamine metabolism, is a potential target for therapies to overcome these diseases. Eukaryotic phylogenies were constructed from full-length genes for ODC to determine the origin of ODC in the kinetoplastid protozoans. The Odc genes from Trypanosoma brucei and two other African trypanosomes, T. congolense and T. vivax, clustered with Odc genes from vertebrates rather than with Odc genes from other kinetoplastids and other protozoans, making this gene a candidate for horizontal gene transfer from a vertebrate source. This result is unique to the Odc gene from the African trypanosomes as four other genes produced phylogenies consistent with the expected taxonomic relationships for the organisms. Analysis of the genomic regions around the Odc genes in Leishmania major, T. brucei, and Trypanosoma cruzi supports the hypothesis of loss of the Odc gene in the Trypanosoma lineage followed by acquisition of a new copy from a vertebrate host in the African branch of the genus.
AB - Kinetoplastid protozoans in the family Trypanosomatidae are parasites, many of them responsible for serious diseases in humans and domestic animals. Ornithine decarboxlyase (ODC), a protein at the core of polyamine metabolism, is a potential target for therapies to overcome these diseases. Eukaryotic phylogenies were constructed from full-length genes for ODC to determine the origin of ODC in the kinetoplastid protozoans. The Odc genes from Trypanosoma brucei and two other African trypanosomes, T. congolense and T. vivax, clustered with Odc genes from vertebrates rather than with Odc genes from other kinetoplastids and other protozoans, making this gene a candidate for horizontal gene transfer from a vertebrate source. This result is unique to the Odc gene from the African trypanosomes as four other genes produced phylogenies consistent with the expected taxonomic relationships for the organisms. Analysis of the genomic regions around the Odc genes in Leishmania major, T. brucei, and Trypanosoma cruzi supports the hypothesis of loss of the Odc gene in the Trypanosoma lineage followed by acquisition of a new copy from a vertebrate host in the African branch of the genus.
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U2 - 10.1016/j.meegid.2005.05.004
DO - 10.1016/j.meegid.2005.05.004
M3 - Article
C2 - 16344004
AN - SCOPUS:33645580051
SN - 1567-1348
VL - 6
SP - 205
EP - 219
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 3
ER -
