The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences

Tanja M. Gruber; Jonathan A. Eisen; Kurt Gish; Donald A. Bryant

doi:10.1016/S0378-1097(98)00102-5

The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences

Tanja M. Gruber, Jonathan A. Eisen, Kurt Gish, Donald A. Bryant

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram- positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.

Original language	English (US)
Pages (from-to)	53-60
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	162
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(98)00102-5
State	Published - May 1 1998

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(98)00102-5

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@article{48157f9886a14a65abea06edb376d53a,

title = "The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences",

abstract = "Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram- positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.",

author = "Gruber, {Tanja M.} and Eisen, {Jonathan A.} and Kurt Gish and Bryant, {Donald A.}",

note = "Funding Information: This work was supported by USPHS grant GM-31625 and DOE grant DE-FG02-94ER20137 to D.A.B. In addition, J.A.E. was supported in part by Outstanding Investigator Grant CA44349 from the National Cancer Institute to Philip C. Hanawalt. We would also like to thank Alberto I. Roca for help in tracking down unpublished RecA sequences and Rob Wheeler for sharing the Caulobacter crescentus RecA sequence prior to publication.",

year = "1998",

month = may,

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doi = "10.1016/S0378-1097(98)00102-5",

language = "English (US)",

volume = "162",

pages = "53--60",

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T1 - The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences

AU - Gruber, Tanja M.

AU - Eisen, Jonathan A.

AU - Gish, Kurt

AU - Bryant, Donald A.

N1 - Funding Information: This work was supported by USPHS grant GM-31625 and DOE grant DE-FG02-94ER20137 to D.A.B. In addition, J.A.E. was supported in part by Outstanding Investigator Grant CA44349 from the National Cancer Institute to Philip C. Hanawalt. We would also like to thank Alberto I. Roca for help in tracking down unpublished RecA sequences and Rob Wheeler for sharing the Caulobacter crescentus RecA sequence prior to publication.

PY - 1998/5/1

Y1 - 1998/5/1

N2 - Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram- positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.

AB - Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram- positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.

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The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences

Abstract

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