TY - JOUR
T1 - The plasmid replicon of Epstein-Barr virus
T2 - Mechanistic insights into efficient, licensed, extrachromosomal replication in human cells
AU - Lindner, Scott E.
AU - Sugden, Bill
N1 - Funding Information:
We thank Chen-Yu Wang for providing her unpublished findings. We thank too the members of the Sugden lab for critically reviewing this manuscript. Our work has been supported by grants from the NIH: CA22443 and T32CA09135. B.S. is an American Cancer Society Research Professor.
PY - 2007/7
Y1 - 2007/7
N2 - The genome of Epstein-Barr Virus (EBV) and plasmid derivatives of it are among the most efficient extrachromosomal replicons in mammalian cells. The latent origin of plasmid replication (oriP), when supplied with the viral Epstein-Barr Nuclear Antigen 1 (EBNA1) in trans, provides efficient duplication, partitioning and maintenance of plasmids bearing it. In this review, we detail what is known about the viral cis and trans elements required for plasmid replication. In addition, we describe how the cellular factors that EBV usurps are used to complement the functions of the viral constituents. Finally, we propose a model for the sequential assembly of an EBNA1-dependent origin of DNA synthesis into a pre-Replicative Complex (pre-RC), which functions by making use only of cellular enzymatic activities to carry out the replication of the viral plasmid.
AB - The genome of Epstein-Barr Virus (EBV) and plasmid derivatives of it are among the most efficient extrachromosomal replicons in mammalian cells. The latent origin of plasmid replication (oriP), when supplied with the viral Epstein-Barr Nuclear Antigen 1 (EBNA1) in trans, provides efficient duplication, partitioning and maintenance of plasmids bearing it. In this review, we detail what is known about the viral cis and trans elements required for plasmid replication. In addition, we describe how the cellular factors that EBV usurps are used to complement the functions of the viral constituents. Finally, we propose a model for the sequential assembly of an EBNA1-dependent origin of DNA synthesis into a pre-Replicative Complex (pre-RC), which functions by making use only of cellular enzymatic activities to carry out the replication of the viral plasmid.
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U2 - 10.1016/j.plasmid.2007.01.003
DO - 10.1016/j.plasmid.2007.01.003
M3 - Review article
C2 - 17350094
AN - SCOPUS:34249864763
SN - 0147-619X
VL - 58
SP - 1
EP - 12
JO - Plasmid
JF - Plasmid
IS - 1
ER -