TY - JOUR
T1 - The psaC genes of Synechococcus sp. PCC7002 and Cyanophora paradoxa
T2 - cloning and sequence analysis
AU - Rhiel, Erhard
AU - Stirewalt, Veronica L.
AU - Gasparich, Gail E.
AU - Bryant, Donald A.
N1 - Funding Information:
This work was supported by NSF grants DMB-8504294 and DMB-S818997 and Pennsylvania State University Experiment Station Project 2874 to D.A.B.E.R. was the recipient of a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft.
PY - 1992/3/1
Y1 - 1992/3/1
N2 - The psaC genes of the cyanobacterium, Synechococcus sp. PCC7002, and of the cyanelle genome of the phylogenetically ambiguous biflagellate, Cyanophora paradoxa, were cloned, mapped and sequenced. The PsaC proteins of both species exhibit high degrees (approx. 95%) of sequence similarity to the PsaC proteins of other cyanobacteria as well as the chloroplast-encoded proteins of green algae and higher plants. The Synechococcus sp. PCC7002 psaC gene is transcribed as a monocistronic mRNA of approx. 350-400 nt, and transcription is initiated 51 nt upstream from the translational start codon. As found for the chloroplasts of higher plants, the C. paradoxa psaC gene is encoded within the small single-copy region of the cyanelle genome. In contrast to results obtained for chloroplasts and for the cyanobacterium Synechocystis sp. PCC6803, neither psaC gene is flanked by genes encoding components of the NAD(P)H dehydrogenase complex.
AB - The psaC genes of the cyanobacterium, Synechococcus sp. PCC7002, and of the cyanelle genome of the phylogenetically ambiguous biflagellate, Cyanophora paradoxa, were cloned, mapped and sequenced. The PsaC proteins of both species exhibit high degrees (approx. 95%) of sequence similarity to the PsaC proteins of other cyanobacteria as well as the chloroplast-encoded proteins of green algae and higher plants. The Synechococcus sp. PCC7002 psaC gene is transcribed as a monocistronic mRNA of approx. 350-400 nt, and transcription is initiated 51 nt upstream from the translational start codon. As found for the chloroplasts of higher plants, the C. paradoxa psaC gene is encoded within the small single-copy region of the cyanelle genome. In contrast to results obtained for chloroplasts and for the cyanobacterium Synechocystis sp. PCC6803, neither psaC gene is flanked by genes encoding components of the NAD(P)H dehydrogenase complex.
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U2 - 10.1016/0378-1119(92)90313-E
DO - 10.1016/0378-1119(92)90313-E
M3 - Article
C2 - 1551590
AN - SCOPUS:0026581091
SN - 0378-1119
VL - 112
SP - 123
EP - 128
JO - Gene
JF - Gene
IS - 1
ER -