The receptor tyrosine kinase Ror is required for dendrite regeneration in Drosophila neurons

Derek M.R. Nye, Richard M. Albertson, Alexis T. Weiner, J. Ian Hertzler, Matthew Shorey, Deborah C.I. Goberdhan, Clive Wilson, Kevin A. Janes, Melissa M. Rolls

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


While many regulators of axon regeneration have been identified, very little is known about mechanisms that allow dendrites to regenerate after injury. Using a Drosophila model of dendrite regeneration, we performed a candidate screen of receptor tyrosine kinases (RTKs) and found a requirement for RTK-like orphan receptor (Ror). We confirmed that Ror was required for regeneration in two different neuron types using RNA interference (RNAi) and mutants. Ror was not required for axon regeneration or normal dendrite development, suggesting a specific role in dendrite regeneration. Ror can act as a Wnt coreceptor with frizzleds (fzs) in other contexts, so we tested the involvement of Wnt signaling proteins in dendrite regeneration. We found that knockdown of fz, dishevelled (dsh), Axin, and gilgamesh (gish) also reduced dendrite regeneration. Moreover, Ror was required to position dsh and Axin in dendrites. We recently found that Wnt signaling proteins, including dsh and Axin, localize microtubule nucleation machinery in dendrites. We therefore hypothesized that Ror may act by regulating microtubule nucleation at baseline and during dendrite regeneration. Consistent with this hypothesis, localization of the core nucleation protein γTubulin was reduced in Ror RNAi neurons, and this effect was strongest during dendrite regeneration. In addition, dendrite regeneration was sensitive to partial reduction of γTubulin. We conclude that Ror promotes dendrite regeneration as part of a Wnt signaling pathway that regulates dendritic microtubule nucleation.

Original languageEnglish (US)
Article numbere3000657
JournalPLoS biology
Issue number3
StatePublished - Mar 2020

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Agricultural and Biological Sciences


Dive into the research topics of 'The receptor tyrosine kinase Ror is required for dendrite regeneration in Drosophila neurons'. Together they form a unique fingerprint.

Cite this