@inbook{79dc8a1f7a534adaa16a7f24fc1cee83,
title = "The ribosome binding site calculator",
abstract = "The Ribosome Binding Site (RBS) Calculator is a design method for predicting and controlling translation initiation and protein expression in bacteria. The method can predict the rate of translation initiation for every start codon in an mRNA transcript. The method may also optimize a synthetic RBS sequence to achieve a targeted translation initiation rate. Using the RBS Calculator, a protein coding sequence's translation rate may be rationally controlled across a 100,000+ fold range. We begin by providing an overview of the potential biotechnology applications of the RBS Calculator, including the optimization of synthetic metabolic pathways and genetic circuits. We then detail the definitions, methodologies, and algorithms behind the RBS Calculator's thermodynamic model and optimization method. Finally, we outline a protocol for precisely measuring steady-state fluorescent protein expression levels. These methods and protocols provide a clear explanation of the RBS Calculator and its uses.",
author = "Salis, {Howard M.}",
note = "Funding Information: We would like to thank Joseph Wade (Wadsworth Center) for an insightful discussion on genome-scale translation rates. This work was supported by a DARPA Young Faculty Award. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2011",
doi = "10.1016/B978-0-12-385120-8.00002-4",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "19--42",
booktitle = "Methods in Enzymology",
address = "United States",
}