The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Haijun Zhao; Xueyan Wang; Yulin Jia; Bastian Minkenberg; Matthew Wheatley; Jiangbo Fan; Melissa H. Jia; Adam Famoso; Jeremy D. Edwards; Yeshi Wamishe; Barbara Valent; Guo Liang Wang; Yinong Yang

doi:10.1038/s41467-018-04369-4

The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Haijun Zhao, Xueyan Wang, Yulin Jia, Bastian Minkenberg, Matthew Wheatley, Jiangbo Fan, Melissa H. Jia, Adam Famoso, Jeremy D. Edwards, Yeshi Wamishe, Barbara Valent, Guo Liang Wang, Yinong Yang

Research output: Contribution to journal › Article › peer-review

155 Scopus citations

Abstract

Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

Original language	English (US)
Article number	2039
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-04369-4
State	Published - Dec 1 2018

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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title = "The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance",

abstract = "Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.",

author = "Haijun Zhao and Xueyan Wang and Yulin Jia and Bastian Minkenberg and Matthew Wheatley and Jiangbo Fan and Jia, {Melissa H.} and Adam Famoso and Edwards, {Jeremy D.} and Yeshi Wamishe and Barbara Valent and Wang, {Guo Liang} and Yinong Yang",

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doi = "10.1038/s41467-018-04369-4",

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T1 - The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

AU - Zhao, Haijun

AU - Wang, Xueyan

AU - Jia, Yulin

AU - Minkenberg, Bastian

AU - Wheatley, Matthew

AU - Fan, Jiangbo

AU - Jia, Melissa H.

AU - Famoso, Adam

AU - Edwards, Jeremy D.

AU - Wamishe, Yeshi

AU - Valent, Barbara

AU - Wang, Guo Liang

AU - Yang, Yinong

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

AB - Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

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The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Abstract

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