The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain

Matthew L. Baker; Irina I. Serysheva; Serap Sencer; Yili Wu; Steven J. Ludtke; Wen Jiang; Susan L. Hamilton; Wah Chiu

doi:10.1073/pnas.182058899

The skeletal muscle Ca²⁺ release channel has an oxidoreductase-like domain

Matthew L. Baker, Irina I. Serysheva, Serap Sencer, Yili Wu, Steven J. Ludtke, Wen Jiang, Susan L. Hamilton, Wah Chiu

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Abstract

We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca²⁺ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41-420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22A structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [³H]NAD⁺, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.

Original language	English (US)
Pages (from-to)	12155-12160
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	19
DOIs	https://doi.org/10.1073/pnas.182058899
State	Published - Sep 17 2002

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title = "The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain",

abstract = "We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca2+ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41-420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22A structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.",

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T1 - The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain

AU - Baker, Matthew L.

AU - Serysheva, Irina I.

AU - Sencer, Serap

AU - Wu, Yili

AU - Ludtke, Steven J.

AU - Jiang, Wen

AU - Hamilton, Susan L.

AU - Chiu, Wah

PY - 2002/9/17

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AB - We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca2+ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41-420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22A structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.

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The skeletal muscle Ca²⁺ release channel has an oxidoreductase-like domain

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