TY - JOUR
T1 - The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB
AU - Taggart, Andrew K.P.
AU - Fisher, Timothy S.
AU - Pugh, B. Franklin
N1 - Funding Information:
We are extremely grateful to Bob White and Steve Jackson for providing the pol Ill templates. We thank Susan Abmayr, Dave Gilmour, Steve Hahn, and Bob Roeder for many helpful comments. This work was supported by grants from the Pennsylvania State University, the Searle Scholars Program/the Chicago Community Trust, and the National Institutes of Health GM47655-01.
PY - 1992/12/11
Y1 - 1992/12/11
N2 - RNA polymerases I, II, and III require the TATA-binding protein (TBP) to initiate promoter-specific transcription. We have separated HeLa TBP into four phosphocellulose fractions that elicit polymerase specificity in supplying TBP activity to TBP-depleted pol II and pol III transcription reactions. Polymerase specificity might arise in part through distinct TBP-associated factors (TAFs), which have recently been identified in pol I and II transcription. However, the requirement for pol III TAFs has not been established. Here we show that classical pol III transcription involves a minimum of two novel TAFs: TAF-172 and TAF-L. Not only does TAF-172 activate pol III transcription, but it also inhibits the binding of TBP to the TATA box, thereby repressing pol II transcription. The TBP-TAF-172-TAF-L complex can replace TFIIIB both in transcription reactions reconstituted with TFIIIC and in template commitment assays. Thus SL1, TFIID, and TFIIIB might be functionally similar TBP-TAF complexes that direct pol I, II, and III transcription, respectively.
AB - RNA polymerases I, II, and III require the TATA-binding protein (TBP) to initiate promoter-specific transcription. We have separated HeLa TBP into four phosphocellulose fractions that elicit polymerase specificity in supplying TBP activity to TBP-depleted pol II and pol III transcription reactions. Polymerase specificity might arise in part through distinct TBP-associated factors (TAFs), which have recently been identified in pol I and II transcription. However, the requirement for pol III TAFs has not been established. Here we show that classical pol III transcription involves a minimum of two novel TAFs: TAF-172 and TAF-L. Not only does TAF-172 activate pol III transcription, but it also inhibits the binding of TBP to the TATA box, thereby repressing pol II transcription. The TBP-TAF-172-TAF-L complex can replace TFIIIB both in transcription reactions reconstituted with TFIIIC and in template commitment assays. Thus SL1, TFIID, and TFIIIB might be functionally similar TBP-TAF complexes that direct pol I, II, and III transcription, respectively.
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U2 - 10.1016/0092-8674(92)90396-T
DO - 10.1016/0092-8674(92)90396-T
M3 - Article
C2 - 1458533
AN - SCOPUS:0026497016
SN - 0092-8674
VL - 71
SP - 1015
EP - 1028
JO - Cell
JF - Cell
IS - 6
ER -