Abstract
A simple technique for differentiation of herpes simplex virus type 1 from type 2, using growth variations in tissue culture and temperature sensitivity is described. All HSV-2 isolates were capable of producing distinct microplaques in chick embryo cells while the newly isolated HSV-1 strains failed to do so. Newly isolated HSV-1 strains showed similar or slightly lower titers in rabbit kidney when incubated at 35 or 40°. In contrast, the HSV-2 isolates generally showed distinctly lower titers or delayed CPE at 40°. Virus typings by selective growth in cell culture and temperature markers were confirmed by neutralization and/or immunofluorescence tests. Since both types of HSV can be isolated from any site of the body, the availability of a simple laboratory technique for distinguishing between the two provides physicians and diagnostic virology laboratories with a means for rapid and accurate diagnosis of herpetic infections.
Original language | English (US) |
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Pages (from-to) | 118-123 |
Number of pages | 6 |
Journal | Proceedings of the Society for Experimental Biology and Medicine |
Volume | 155 |
Issue number | 1 |
DOIs | |
State | Published - May 1977 |
All Science Journal Classification (ASJC) codes
- General Biochemistry, Genetics and Molecular Biology