The V599E BRAF mutation is uncommon in biliary tract cancers

David Goldenberg, Eli Rosenbaum, Pedram Argani, Ignacio I. Wistuba, David Sidransky, Paul J. Thuluvath, Manuel Hidalgo, Joseph Califano, Anirban Maitra

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Activating point mutations of the BRAF oncogene have been identified in several solid tumors, most commonly in cutaneous melanomas and papillary carcinomas of the thyroid. A specific point mutation-V599E-accounts for the overwhelming majority of these mutational events. We explored the frequency of the V599E BRAF mutation in biliary tract cancers, in all, 62 archival biliary tract cancers, including 15 gallbladder cancers, 15 extrahepatic, and 10 intrahepatic cholangiocarcinomas from the United States, and 22 gallbladder carcinomas from Chile were analysed for the V599E mutation of the BRAF gene using three distinct methods: direct sequencing, a primer extension method (Mutector® assay), and the highly sensitive quantitative Gap Ligase Chain Reaction. The common V599E mutation was not identified in any of the 62 biliary cancer samples using these three methods of detection. The V599E somatic mutation of the BRAF gene is absent in biliary tract cancers, at least in the two geographic populations (United States and Chile) examined. Activation of the RAS/RAF/MAP kinase pathway in biliary tract cancers is likely to be secondary to oncogenic RAS mutations, or due to mutations of the BRAF gene at nucleotide positions not explored in the current study.

Original languageEnglish (US)
Pages (from-to)1386-1391
Number of pages6
JournalModern Pathology
Issue number11
StatePublished - Nov 2004

All Science Journal Classification (ASJC) codes

  • General Medicine


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