The yeast plasmid 2μ circle encodes components required for its high copy propagation

M. Jayaram; Y. Y. Li; J. R. Broach

doi:10.1016/0092-8674(83)90139-3

The yeast plasmid 2μ circle encodes components required for its high copy propagation

M. Jayaram, Y. Y. Li, J. R. Broach

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

The yeast plasmid 2μ and certain hybrid plasmids constructed from it are maintained stably and at high copy number in yeast cells. By examining various mutant hybrid 2μ plasmids, we show that these properties require the integrity of four plasmid loci. Two of these, designated REPI and REP2, are active in trans and correspond to two open coding regions of 2μ. The other two loci are active only in cis and correspond to the origin of replication and to a region, designated REP3, located several hundred bp away from the origin and consisting of direct repeats of a 62 bp sequence. We propose that the REP loci constitute a copy control system that overrides normal cellular restriction on plasmid replication and amplifies the plasmid when copy number is low.

Original language	English (US)
Pages (from-to)	95-104
Number of pages	10
Journal	Cell
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(83)90139-3
State	Published - Aug 1983

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/0092-8674(83)90139-3

Cite this

@article{302b97f9d4984969a7d52ce6e8328060,

title = "The yeast plasmid 2μ circle encodes components required for its high copy propagation",

abstract = "The yeast plasmid 2μ and certain hybrid plasmids constructed from it are maintained stably and at high copy number in yeast cells. By examining various mutant hybrid 2μ plasmids, we show that these properties require the integrity of four plasmid loci. Two of these, designated REPI and REP2, are active in trans and correspond to two open coding regions of 2μ. The other two loci are active only in cis and correspond to the origin of replication and to a region, designated REP3, located several hundred bp away from the origin and consisting of direct repeats of a 62 bp sequence. We propose that the REP loci constitute a copy control system that overrides normal cellular restriction on plasmid replication and amplifies the plasmid when copy number is low.",

author = "M. Jayaram and Li, {Y. Y.} and Broach, {J. R.}",

note = "Funding Information: We would like to thank Ms. S. lsmael for excellent technical assistance. This work was scpported in part by a National Institutes of Health grant to J. FL B. Y. Y. L. was a recipient of a Lee Hysan fellowship frcm the Committee on Educational Exchange with China. J. R. B. is an Establii Investigator of the American Heart Association.",

year = "1983",

month = aug,

doi = "10.1016/0092-8674(83)90139-3",

language = "English (US)",

volume = "34",

pages = "95--104",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - The yeast plasmid 2μ circle encodes components required for its high copy propagation

AU - Jayaram, M.

AU - Li, Y. Y.

AU - Broach, J. R.

N1 - Funding Information: We would like to thank Ms. S. lsmael for excellent technical assistance. This work was scpported in part by a National Institutes of Health grant to J. FL B. Y. Y. L. was a recipient of a Lee Hysan fellowship frcm the Committee on Educational Exchange with China. J. R. B. is an Establii Investigator of the American Heart Association.

PY - 1983/8

Y1 - 1983/8

N2 - The yeast plasmid 2μ and certain hybrid plasmids constructed from it are maintained stably and at high copy number in yeast cells. By examining various mutant hybrid 2μ plasmids, we show that these properties require the integrity of four plasmid loci. Two of these, designated REPI and REP2, are active in trans and correspond to two open coding regions of 2μ. The other two loci are active only in cis and correspond to the origin of replication and to a region, designated REP3, located several hundred bp away from the origin and consisting of direct repeats of a 62 bp sequence. We propose that the REP loci constitute a copy control system that overrides normal cellular restriction on plasmid replication and amplifies the plasmid when copy number is low.

AB - The yeast plasmid 2μ and certain hybrid plasmids constructed from it are maintained stably and at high copy number in yeast cells. By examining various mutant hybrid 2μ plasmids, we show that these properties require the integrity of four plasmid loci. Two of these, designated REPI and REP2, are active in trans and correspond to two open coding regions of 2μ. The other two loci are active only in cis and correspond to the origin of replication and to a region, designated REP3, located several hundred bp away from the origin and consisting of direct repeats of a 62 bp sequence. We propose that the REP loci constitute a copy control system that overrides normal cellular restriction on plasmid replication and amplifies the plasmid when copy number is low.

UR - http://www.scopus.com/inward/record.url?scp=0020807834&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020807834&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(83)90139-3

DO - 10.1016/0092-8674(83)90139-3

M3 - Article

C2 - 6883512

AN - SCOPUS:0020807834

SN - 0092-8674

VL - 34

SP - 95

EP - 104

JO - Cell

JF - Cell

IS - 1

ER -

The yeast plasmid 2μ circle encodes components required for its high copy propagation

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this