@article{cb0e0863df574f7188a2e304d6f110d5,
title = "Thermally-controlled extrusion-based bioprinting of collagen",
abstract = "Thermally-crosslinked hydrogels in bioprinting have gained increasing attention due to their ability to undergo tunable crosslinking by modulating the temperature and time of crosslinking. In this paper, we present a new bioink composed of collagen type-I and Pluronic{\textregistered} F-127 hydrogels, which was bioprinted using a thermally-controlled bioprinting unit. Bioprintability and rheology of the composite bioink was studied in a thorough manner in order to determine the optimal bioprinting time and extrusion profile of the bioink for fabrication of three-dimensional (3D) constructs, respectively. It was observed that collagen fibers aligned themselves along the directions of the printed filaments after bioprinting based on the results on an anisotropy study. Furthermore, rat bone marrow-derived stem cells (rBMSCs) were bioprinted in order to determine the effect of thermally-controlled extrusion process. In vitro viability and proliferation study revealed that rBMSCs were able to maintain their viability after extrusion and attached to collagen fibers, spread and proliferated within the constructs up to seven days of culture. [Figure not available: see fulltext.].",
author = "Moncal, {Kazim K.} and Veli Ozbolat and Pallab Datta and Heo, {Dong N.} and Ozbolat, {Ibrahim T.}",
note = "Funding Information: Acknowledgements This work was supported by the National Science Foundation Award (CMMI 1462232) and Osteology Foundation Award # 15–042. Dr. Veli Ozbolat acknowledges the support from the International Postdoctoral Research Scholarship Program (BIDEP 2219) of the Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank Dr. Albert Ratner (Mechanical and Industrial Engineering Department, University of Iowa, Iowa City, USA) providing the FLIR thermal camera system. The authors would like to thank Ethan M. Gerhard, Dr. Jian Yang and Kevin P. Godzik (Department of Biomedical Engineering, Penn State University, University Park, USA) for their assistance with the mechanical testing and the rheology study, respectively. The authors would like to also thank Mrs. Alyssa Sipos with her assistance to PSR and immunofluorescence staining. There has been no significant financial support for this work that could have influenced its outcome. Funding Information: This work was supported by the National Science Foundation Award (CMMI 1462232) and Osteology Foundation Award # 15–042. Dr. Veli Ozbolat acknowledges the support from the International Postdoctoral Research Scholarship Program (BIDEP 2219) of the Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank Dr. Albert Ratner (Mechanical and Industrial Engineering Department, University of Iowa, Iowa City, USA) providing the FLIR thermal camera system. The authors would like to thank Ethan M. Gerhard, Dr. Jian Yang and Kevin P. Godzik (Department of Biomedical Engineering, Penn State University, University Park, USA) for their assistance with the mechanical testing and the rheology study, respectively. The authors would like to also thank Mrs. Alyssa Sipos with her assistance to PSR and immunofluorescence staining. There has been no significant financial support for this work that could have influenced its outcome.",
year = "2019",
month = may,
day = "1",
doi = "10.1007/s10856-019-6258-2",
language = "English (US)",
volume = "30",
journal = "Journal of Materials Science: Materials in Medicine",
issn = "0957-4530",
publisher = "Springer New York",
number = "5",
}