Three-dimensional bioprinting using self-Assembling scalable scaffold-free "tissue strands" as a new bioink

Yin Yu, Kazim K. Moncal, Jianqiang Li, Weijie Peng, Iris Rivero, James A. Martin, Ibrahim T. Ozbolat

Research output: Contribution to journalArticlepeer-review

215 Scopus citations


Recent advances in bioprinting have granted tissue engineers the ability to assemble biomaterials, cells, and signaling molecules into anatomically relevant functional tissues or organ parts. Scaffold-free fabrication has recently attracted a great deal of interest due to the ability to recapitulate tissue biology by using self-Assembly, which mimics the embryonic development process. Despite several attempts, bioprinting of scale-up tissues at clinically-relevant dimensions with closely recapitulated tissue biology and functionality is still a major roadblock. Here, we fabricate and engineer scaffold-free scalable tissue strands as a novel bioink material for robotic-Assisted bioprinting technologies. Compare to 400 μm-Thick tissue spheroids bioprinted in a liquid delivery medium into confining molds, near 8 cm-long tissue strands with rapid fusion and self-Assemble capabilities are bioprinted in solid form for the first time without any need for a scaffold or a mold support or a liquid delivery medium, and facilitated native-like scale-up tissues. The prominent approach has been verified using cartilage strands as building units to bioprint articular cartilage tissue.

Original languageEnglish (US)
Article number28714
JournalScientific reports
StatePublished - Jun 27 2016

All Science Journal Classification (ASJC) codes

  • General


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