TY - JOUR
T1 - Three-dimensional manipulation of single cells using surface acoustic waves
AU - Guo, Feng
AU - Mao, Zhangming
AU - Chen, Yuchao
AU - Xie, Zhiwei
AU - Lata, James P.
AU - Li, Peng
AU - Ren, Liqiang
AU - Liu, Jiayang
AU - Yang, Jian
AU - Dao, Ming
AU - Suresh, Subra
AU - Huang, Tony Jun
N1 - Funding Information:
We thank Dr. John Mai for helpful discussions. We also acknowledge financial support from NIH Grants 1R33EB019785-01 and 1 R01 GM112048-01A1, the National Science Foundation, and the Penn State Center for Nanoscale Science (Materials Research Science and Engineering Center) under Grant DMR-0820404. M.D. also acknowledges partial support from NIH Grant U01HL114476.
PY - 2016/2/9
Y1 - 2016/2/9
N2 - The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving "acoustic tweezers" in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.
AB - The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving "acoustic tweezers" in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.
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U2 - 10.1073/pnas.1524813113
DO - 10.1073/pnas.1524813113
M3 - Article
C2 - 26811444
AN - SCOPUS:84957831139
SN - 0027-8424
VL - 113
SP - 1522
EP - 1527
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -