Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology

Christopher Siedlecki; Brian J. Lestini; Steven J. Eppell; Kandice Kottke-Marchant; David Wilson; Roger E. Marchant

Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology

Christopher Siedlecki, Brian J. Lestini, Steven J. Eppell, Kandice Kottke-Marchant, David Wilson, Roger E. Marchant

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The 3-dimensional structure of protein was investigated as well as the effect of conformational changes in the von Willebrand Factor (vWF) on protein function. The effects of applied shear were studied using different atomic force microscopy (AFM) imaging models, while mathematical morphology was used to generate a model of an adsorbed vWG dimer and to predict AFM images. It was shown that submolecular three-dimensional resolution of vWF can be obtained in aqueous solutions using both contact mode and fluid-tapping mode. In the absence of applied shear, vWF adsorbs in a large globular conformation. Applied shear leads to the unraveling of the protein and exposure of the individual domains.

Original language	English (US)
Title of host publication	Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium
Editors	Anon
Publisher	Soc for Biomaterials
Pages	651
Number of pages	1
Volume	1
State	Published - 1996
Event	Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2) - Toronto, Can Duration: May 29 1996 → Jun 2 1996

Other

Other	Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2)
City	Toronto, Can
Period	5/29/96 → 6/2/96

All Science Journal Classification (ASJC) codes

General Materials Science

Cite this

Siedlecki, C., Lestini, B. J., Eppell, S. J., Kottke-Marchant, K., Wilson, D., & Marchant, R. E. (1996). Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology. In Anon (Ed.), Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium (Vol. 1, pp. 651). Soc for Biomaterials.

Siedlecki, Christopher ; Lestini, Brian J. ; Eppell, Steven J. et al. / Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. editor / Anon. Vol. 1 Soc for Biomaterials, 1996. pp. 651

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title = "Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology",

abstract = "The 3-dimensional structure of protein was investigated as well as the effect of conformational changes in the von Willebrand Factor (vWF) on protein function. The effects of applied shear were studied using different atomic force microscopy (AFM) imaging models, while mathematical morphology was used to generate a model of an adsorbed vWG dimer and to predict AFM images. It was shown that submolecular three-dimensional resolution of vWF can be obtained in aqueous solutions using both contact mode and fluid-tapping mode. In the absence of applied shear, vWF adsorbs in a large globular conformation. Applied shear leads to the unraveling of the protein and exposure of the individual domains.",

author = "Christopher Siedlecki and Lestini, {Brian J.} and Eppell, {Steven J.} and Kandice Kottke-Marchant and David Wilson and Marchant, {Roger E.}",

year = "1996",

language = "English (US)",

volume = "1",

pages = "651",

editor = "Anon",

booktitle = "Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium",

publisher = "Soc for Biomaterials",

note = "Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2) ; Conference date: 29-05-1996 Through 02-06-1996",

}

Siedlecki, C, Lestini, BJ, Eppell, SJ, Kottke-Marchant, K, Wilson, D & Marchant, RE 1996, Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology. in Anon (ed.), Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. vol. 1, Soc for Biomaterials, pp. 651, Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2), Toronto, Can, 5/29/96.

Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology. / Siedlecki, Christopher; Lestini, Brian J.; Eppell, Steven J. et al.
Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. ed. / Anon. Vol. 1 Soc for Biomaterials, 1996. p. 651.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology

AU - Siedlecki, Christopher

AU - Lestini, Brian J.

AU - Eppell, Steven J.

AU - Kottke-Marchant, Kandice

AU - Wilson, David

AU - Marchant, Roger E.

PY - 1996

Y1 - 1996

N2 - The 3-dimensional structure of protein was investigated as well as the effect of conformational changes in the von Willebrand Factor (vWF) on protein function. The effects of applied shear were studied using different atomic force microscopy (AFM) imaging models, while mathematical morphology was used to generate a model of an adsorbed vWG dimer and to predict AFM images. It was shown that submolecular three-dimensional resolution of vWF can be obtained in aqueous solutions using both contact mode and fluid-tapping mode. In the absence of applied shear, vWF adsorbs in a large globular conformation. Applied shear leads to the unraveling of the protein and exposure of the individual domains.

AB - The 3-dimensional structure of protein was investigated as well as the effect of conformational changes in the von Willebrand Factor (vWF) on protein function. The effects of applied shear were studied using different atomic force microscopy (AFM) imaging models, while mathematical morphology was used to generate a model of an adsorbed vWG dimer and to predict AFM images. It was shown that submolecular three-dimensional resolution of vWF can be obtained in aqueous solutions using both contact mode and fluid-tapping mode. In the absence of applied shear, vWF adsorbs in a large globular conformation. Applied shear leads to the unraveling of the protein and exposure of the individual domains.

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M3 - Conference contribution

AN - SCOPUS:0030401583

VL - 1

SP - 651

BT - Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium

A2 - Anon, null

PB - Soc for Biomaterials

T2 - Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2)

Y2 - 29 May 1996 through 2 June 1996

ER -

Siedlecki C, Lestini BJ, Eppell SJ, Kottke-Marchant K, Wilson D, Marchant RE. Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology. In Anon, editor, Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 1. Soc for Biomaterials. 1996. p. 651

Three-dimensional model of adsorbed von Willebrand Factor using atomic force microscopy and mathematical morphology

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All Science Journal Classification (ASJC) codes

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