@inbook{c64808a2c26b49be8dac5dbe1c426c6b,
title = "Time-Lapse Imaging of Root Pathogenesis and Fungal Proliferation Without Physically Disrupting Roots",
abstract = "Microscopic observation of root disease onset and progression is typically performed by harvesting different plants at multiple time points. This approach prevents the monitoring of individual encounter sites over time, often mechanically damages roots, and exposes roots to unnatural conditions during observation. Here, we describe a method developed to avoid these problems and its application to study Fusarium oxysporum-Arabidopsis thaliana interactions. This method enabled three-dimensional, time-lapse imaging of both A. thaliana and F. oxysporum as they interact via the use of confocal and multi-photon microscopy and facilitated inquiries about the genetic mechanism underpinning Fusarium wilt.",
author = "Kim, {Hye Seon} and Park, {Sook Young} and Seogchan Kang and Czymmek, {Kirk J.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1795-3_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--170",
booktitle = "Methods in Molecular Biology",
}