Time-Lapse Imaging of Root Pathogenesis and Fungal Proliferation Without Physically Disrupting Roots

Hye Seon Kim, Sook Young Park, Seogchan Kang, Kirk J. Czymmek

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Microscopic observation of root disease onset and progression is typically performed by harvesting different plants at multiple time points. This approach prevents the monitoring of individual encounter sites over time, often mechanically damages roots, and exposes roots to unnatural conditions during observation. Here, we describe a method developed to avoid these problems and its application to study Fusarium oxysporum-Arabidopsis thaliana interactions. This method enabled three-dimensional, time-lapse imaging of both A. thaliana and F. oxysporum as they interact via the use of confocal and multi-photon microscopy and facilitated inquiries about the genetic mechanism underpinning Fusarium wilt.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-170
Number of pages18
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2391
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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