TY - JOUR
T1 - Tissue culture studies of retinal development
AU - Zhang, Samuel Shao Min
AU - Fu, Xin Yuan
AU - Barnstable, Colin J.
N1 - Funding Information:
Original work in this article was supported by NIH Grants EY 00785 (C.J.B.), EY11356 (C.J.B.), EY 13865 (C.J.B.), EY EY13607 (X.Y.F.), and the Allene Reuss Memorial Trust. X.Y.F. is a recipient of Career Development Award (KO4AE01356).
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Because of a limited number of cell types, a series of well-described cell-type-specific markers and a stereotyped sequence of cell development, the retina has been a valuable model of CNS development. Dissociated and explant cultures have been used to help define some of the requirements for differentiation of each major cell class. In addition to mixed-cell cultures it is now possible to use cell purification or selective growth methods to give cultures of single cell types. Alteration of gene expression by viral infection has proved to be a valuable method to help elucidate developmental pathways.
AB - Because of a limited number of cell types, a series of well-described cell-type-specific markers and a stereotyped sequence of cell development, the retina has been a valuable model of CNS development. Dissociated and explant cultures have been used to help define some of the requirements for differentiation of each major cell class. In addition to mixed-cell cultures it is now possible to use cell purification or selective growth methods to give cultures of single cell types. Alteration of gene expression by viral infection has proved to be a valuable method to help elucidate developmental pathways.
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U2 - 10.1016/S1046-2023(02)00263-3
DO - 10.1016/S1046-2023(02)00263-3
M3 - Article
C2 - 12507462
AN - SCOPUS:0037004925
SN - 1046-2023
VL - 28
SP - 439
EP - 447
JO - Methods
JF - Methods
IS - 4
ER -