Abstract
Transgenic mice carrying multiple copies of a recoverable λ phage shuttle vector carrying the supF mutation reporter gene (λsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 × 10-5), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue-specific differences in genetic instability.
Original language | English (US) |
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Pages (from-to) | 459-464 |
Number of pages | 6 |
Journal | Environmental and Molecular Mutagenesis |
Volume | 28 |
Issue number | 4 |
DOIs | |
State | Published - 1996 |
All Science Journal Classification (ASJC) codes
- Epidemiology
- Genetics(clinical)
- Health, Toxicology and Mutagenesis