TY - JOUR
T1 - Tissue targeting and plasma clearance of cobra venom factor in mice
AU - Fu, Qinlan
AU - Satyaswaroop, Pondichery G.
AU - Gowda, D. Channe
N1 - Funding Information:
D.C.G. thanks Dr. Eugene A. Davidson for the support and encouragement. This work was supported by NIH Grant CA61210 to D.C.G.
PY - 1997/2/13
Y1 - 1997/2/13
N2 - The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation with a plasma half life of about 10 h, whereas CVF injected into the peritoneal cavity was slowly absorbed into the blood stream reaching a maximum level at ~ 6 h, and it was then cleared from the circulation with a plasma half life of about 18 h. The rate of plasma clearance of CVF was markedly decreased upon removal of the terminal α-galactosyl residues of the oligosaccharide chains; the plasma half lives for intravenously and intraperitoneally administered de-α-galactosylated CVF were ~ 5 and ~ 10 h, respectively. However, the clearance rate was not affected by complete deglycosylation using N-glycanase or by chemical modification of the terminal galactosyl residues. Together, these data demonstrate that the terminal α-galactosyl residues of CVF mask the Lewis X-dependent uptake of CVF by liver.
AB - The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation with a plasma half life of about 10 h, whereas CVF injected into the peritoneal cavity was slowly absorbed into the blood stream reaching a maximum level at ~ 6 h, and it was then cleared from the circulation with a plasma half life of about 18 h. The rate of plasma clearance of CVF was markedly decreased upon removal of the terminal α-galactosyl residues of the oligosaccharide chains; the plasma half lives for intravenously and intraperitoneally administered de-α-galactosylated CVF were ~ 5 and ~ 10 h, respectively. However, the clearance rate was not affected by complete deglycosylation using N-glycanase or by chemical modification of the terminal galactosyl residues. Together, these data demonstrate that the terminal α-galactosyl residues of CVF mask the Lewis X-dependent uptake of CVF by liver.
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U2 - 10.1006/bbrc.1997.6078
DO - 10.1006/bbrc.1997.6078
M3 - Article
C2 - 9070270
AN - SCOPUS:0031566249
SN - 0006-291X
VL - 231
SP - 316
EP - 320
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -