Tissue targeting and plasma clearance of cobra venom factor in mice

Qinlan Fu, Pondichery G. Satyaswaroop, D. Channe Gowda

    Research output: Contribution to journalArticlepeer-review

    13 Scopus citations

    Abstract

    The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation with a plasma half life of about 10 h, whereas CVF injected into the peritoneal cavity was slowly absorbed into the blood stream reaching a maximum level at ~ 6 h, and it was then cleared from the circulation with a plasma half life of about 18 h. The rate of plasma clearance of CVF was markedly decreased upon removal of the terminal α-galactosyl residues of the oligosaccharide chains; the plasma half lives for intravenously and intraperitoneally administered de-α-galactosylated CVF were ~ 5 and ~ 10 h, respectively. However, the clearance rate was not affected by complete deglycosylation using N-glycanase or by chemical modification of the terminal galactosyl residues. Together, these data demonstrate that the terminal α-galactosyl residues of CVF mask the Lewis X-dependent uptake of CVF by liver.

    Original languageEnglish (US)
    Pages (from-to)316-320
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume231
    Issue number2
    DOIs
    StatePublished - Feb 13 1997

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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