TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes

Andrea M. Schneider, Robert P. Feehan, Mackenzie L. Sennett, Carson A. Wills, Charlotte Garner, Zhaoyuan Cong, Elizabeth M. Billingsley, Alexandra F. Flamm, Lisa M. Shantz, Amanda M. Nelson

Research output: Contribution to journalArticlepeer-review

Abstract

TLR3 is expressed in human skin and keratinocytes, and given its varied role in skin inflammation, development, and regeneration, we sought to determine the cellular response in normal human keratinocytes to TLR3 activation. We investigated this mechanism by treating primary human keratinocytes with both UVB, an endogenous and physiologic TLR3 activator, and poly(I:C), a synthetic and selective TLR3 ligand. TLR3 activation with either UVB or poly(I:C) altered keratinocyte morphology, coinciding with the key features of epithelial-to-mesenchymal transition: increased epithelial-to-mesenchymal transition gene expression, enhanced migration, and increased invasion properties. These results confirm and extend previous studies demonstrating that in addition to its classical role in the innate immune response, TLR3 signaling also regulates stem cell–like properties and developmental programs.

Original languageEnglish (US)
Article numbere202402777
JournalLife Science Alliance
Volume7
Issue number12
DOIs
StatePublished - Dec 2024

All Science Journal Classification (ASJC) codes

  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes'. Together they form a unique fingerprint.

Cite this