@inbook{60ddb91d68dd4dbca17819017d929765,
title = "Toeprint Assays for Detecting RNA Structure and Protein–RNA Interactions",
abstract = "Toeprint assays are primer extension inhibition assays that can detect the 3′ end of an RNA secondary structure, the position of a bound RNA binding protein, as well as the position of a bound 30S ribosomal subunit or a stalled ribosome. Here we describe how this assay was used to identify an RNA hairpin that sequesters a Shine–Dalgarno sequence, how the RNA-binding protein CsrA can alter RNA structure and affect 30S ribosomal subunit binding, and how the macrolide antibiotic tylosin can induce ribosome stalling.",
author = "Helen Yakhnin and Paul Babitzke",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2413-5\_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "305--316",
booktitle = "Methods in Molecular Biology",
}