@inbook{c5ff4a4321a74c25abdaecd9d8ebe5bb,
title = "TonB System, In Vivo Assays and Characterization",
abstract = "The multiprotein TonB system of Escherichia coli involves proteins in both the cytoplasmic membrane and the outer membrane. By a still unclear mechanism, the proton-motive force of the cytoplasmic membrane is used to catalyze active transport through high-affinity transporters in the outer membrane. TonB, ExbB, and ExbD are required to transduce the cytoplasmic membrane energy to these transporters. For E. coli, transport ligands consist of iron-siderophore complexes, vitamin B12, group B colicins, and bacteriophages T1 and {\o}80. Our experimental philosophy is that data gathered in vivo, where all known and unknown components are present at balanced chromosomal levels in the whole cell, can be interpreted with less ambiguity than when a subset of components is overexpressed or analysed in vitro. This chapter describes in vivo assays for the TonB system and their application.",
author = "Kathleen Postle",
note = "Funding Information: I am very grateful to colleagues Volkmar Braun, Clive Bradbeer, Robert Kadner, Joe Neilands, Tony Pugsley, and Peter Reeves for their pioneering work on the TonB system phenotypic assays, which we did not invent, only adapt. Past members of the laboratory, Penelope Higgs, Joydeep Ghosh, Ray Larsen, Tracy Letain, and Jonathan Skare, deserve special recognition for their development of the more mechanistically informative in vivo TonB system assays. I am also grateful to the National Institutes of General Medical Sciences and the National Science Foundation for their support over many years. Finally, I thank Ray Larsen, Marta Manning, Anne Ollis, Kyle Kastead, and Charles Bulathsinghala for critical reading of the manuscript.",
year = "2007",
doi = "10.1016/S0076-6879(06)22012-3",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "245--269",
booktitle = "Two Component Signaling Systems, Part A",
address = "United States",
}