Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics

T. L. King; B. A. Lubinski; M. K. Burnham-Curtis; W. Stott; R. P. Morgan

doi:10.1007/s12686-012-9603-z

Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics

T. L. King
, B. A. Lubinski
, M. K. Burnham-Curtis
, W. Stott
, R. P. Morgan

Ecosystem Science and Management

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

We document isolation and characterization of 13 tri- and tetranucleotide microsatellite DNA markers in brook trout (Salvelinus fontinalis). These markers displayed moderate to high levels of allelic diversity (averaging 20.5 alleles/locus) and heterozygosity (averaging 53.5%) in a range-wide survey of more than 13,000 fish. A comparison of two geographically proximal populations located on opposite sides of the eastern continental divide in Maryland, USA, found no deviations from Hardy-Weinberg equilibrium and minimal linkage disequilibrium. Microsatellite markers developed for S. fontinalis yielded sufficient genetic diversity to: (1) produce unique multilocus genotypes; (2) elucidate phylogeographic structure; and (3) provide unique demographic perspectives of population sizes and historical demographics. This suite of markers also provided considerable cross-species amplification utility among related salmonids.

Original language	English (US)
Pages (from-to)	539-543
Number of pages	5
Journal	Conservation Genetics Resources
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/s12686-012-9603-z
State	Published - Sep 2012

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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King, T. L., Lubinski, B. A., Burnham-Curtis, M. K., Stott, W., & Morgan, R. P. (2012). Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics. Conservation Genetics Resources, 4(3), 539-543. https://doi.org/10.1007/s12686-012-9603-z

King, T. L. ; Lubinski, B. A. ; Burnham-Curtis, M. K. et al. / Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis) : Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics. In: Conservation Genetics Resources. 2012 ; Vol. 4, No. 3. pp. 539-543.

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title = "Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics",

abstract = "We document isolation and characterization of 13 tri- and tetranucleotide microsatellite DNA markers in brook trout (Salvelinus fontinalis). These markers displayed moderate to high levels of allelic diversity (averaging 20.5 alleles/locus) and heterozygosity (averaging 53.5\%) in a range-wide survey of more than 13,000 fish. A comparison of two geographically proximal populations located on opposite sides of the eastern continental divide in Maryland, USA, found no deviations from Hardy-Weinberg equilibrium and minimal linkage disequilibrium. Microsatellite markers developed for S. fontinalis yielded sufficient genetic diversity to: (1) produce unique multilocus genotypes; (2) elucidate phylogeographic structure; and (3) provide unique demographic perspectives of population sizes and historical demographics. This suite of markers also provided considerable cross-species amplification utility among related salmonids.",

author = "King, \{T. L.\} and Lubinski, \{B. A.\} and Burnham-Curtis, \{M. K.\} and W. Stott and Morgan, \{R. P.\}",

note = "Funding Information: Acknowledgments This research was funded by the U.S. Geological Survey{\textquoteright}s (USGS) and National Park Service{\textquoteright}s (NPS) joint Natural Resource Preservation Program (NRPP) with additional funding provided by the USGS Leetown Science Center. The authors wish to thank Roseanna Coleman, Shannon Julian, and Colleen Young for critical laboratory assistance. This article is in partial fulfillment of a final report for NRPP. The authors wish to thank Diane Pavek",

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King, TL, Lubinski, BA, Burnham-Curtis, MK, Stott, W & Morgan, RP 2012, 'Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics', Conservation Genetics Resources, vol. 4, no. 3, pp. 539-543. https://doi.org/10.1007/s12686-012-9603-z

Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics. / King, T. L.; Lubinski, B. A.; Burnham-Curtis, M. K. et al.
In: Conservation Genetics Resources, Vol. 4, No. 3, 09.2012, p. 539-543.

Research output: Contribution to journal › Article › peer-review

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N1 - Funding Information: Acknowledgments This research was funded by the U.S. Geological Survey’s (USGS) and National Park Service’s (NPS) joint Natural Resource Preservation Program (NRPP) with additional funding provided by the USGS Leetown Science Center. The authors wish to thank Roseanna Coleman, Shannon Julian, and Colleen Young for critical laboratory assistance. This article is in partial fulfillment of a final report for NRPP. The authors wish to thank Diane Pavek

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AB - We document isolation and characterization of 13 tri- and tetranucleotide microsatellite DNA markers in brook trout (Salvelinus fontinalis). These markers displayed moderate to high levels of allelic diversity (averaging 20.5 alleles/locus) and heterozygosity (averaging 53.5%) in a range-wide survey of more than 13,000 fish. A comparison of two geographically proximal populations located on opposite sides of the eastern continental divide in Maryland, USA, found no deviations from Hardy-Weinberg equilibrium and minimal linkage disequilibrium. Microsatellite markers developed for S. fontinalis yielded sufficient genetic diversity to: (1) produce unique multilocus genotypes; (2) elucidate phylogeographic structure; and (3) provide unique demographic perspectives of population sizes and historical demographics. This suite of markers also provided considerable cross-species amplification utility among related salmonids.

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King TL, Lubinski BA, Burnham-Curtis MK, Stott W, Morgan RP. Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics. Conservation Genetics Resources. 2012 Sep;4(3):539-543. doi: 10.1007/s12686-012-9603-z

Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics

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