Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope

J. P. Stitt; R. L. Tutwiler; V. Gopalan; L. Larrson

Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope

J. P. Stitt, R. L. Tutwiler, V. Gopalan, L. Larrson

Research output: Contribution to journal › Conference article › peer-review

Abstract

This paper describes the implementation of a wet-cell collection-mode near-field optical microscope that has been constructed with the long term goal of imaging the myosin conformational change as it interacts with actin. The bioassay used to prepare the samples involved lysing muscle fibers to exact and myosin molecules which were fixed to a mica slide. Next, actin filaments were exacted and deposited in the region of the myosin. The wet-cell allows the addition of water containing ATP and the necessary ions to activate the myosin biomolecules. The near-field optical microscope was shown to able to resolve features of the size of the actin and myosin biomolecules.

Original language	English (US)
Pages (from-to)	5250-5253
Number of pages	4
Journal	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	26 VII
State	Published - Dec 1 2004
Event	Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States Duration: Sep 1 2004 → Sep 5 2004

All Science Journal Classification (ASJC) codes

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Cite this

@article{e5b5a4a795c247399a04a21770116eec,

title = "Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope",

abstract = "This paper describes the implementation of a wet-cell collection-mode near-field optical microscope that has been constructed with the long term goal of imaging the myosin conformational change as it interacts with actin. The bioassay used to prepare the samples involved lysing muscle fibers to exact and myosin molecules which were fixed to a mica slide. Next, actin filaments were exacted and deposited in the region of the myosin. The wet-cell allows the addition of water containing ATP and the necessary ions to activate the myosin biomolecules. The near-field optical microscope was shown to able to resolve features of the size of the actin and myosin biomolecules.",

author = "Stitt, {J. P.} and Tutwiler, {R. L.} and V. Gopalan and L. Larrson",

year = "2004",

month = dec,

day = "1",

language = "English (US)",

volume = "26 VII",

pages = "5250--5253",

journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

issn = "0589-1019",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 ; Conference date: 01-09-2004 Through 05-09-2004",

}

Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope. / Stitt, J. P.; Tutwiler, R. L.; Gopalan, V. et al.
In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Vol. 26 VII, 01.12.2004, p. 5250-5253.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope

AU - Stitt, J. P.

AU - Tutwiler, R. L.

AU - Gopalan, V.

AU - Larrson, L.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - This paper describes the implementation of a wet-cell collection-mode near-field optical microscope that has been constructed with the long term goal of imaging the myosin conformational change as it interacts with actin. The bioassay used to prepare the samples involved lysing muscle fibers to exact and myosin molecules which were fixed to a mica slide. Next, actin filaments were exacted and deposited in the region of the myosin. The wet-cell allows the addition of water containing ATP and the necessary ions to activate the myosin biomolecules. The near-field optical microscope was shown to able to resolve features of the size of the actin and myosin biomolecules.

AB - This paper describes the implementation of a wet-cell collection-mode near-field optical microscope that has been constructed with the long term goal of imaging the myosin conformational change as it interacts with actin. The bioassay used to prepare the samples involved lysing muscle fibers to exact and myosin molecules which were fixed to a mica slide. Next, actin filaments were exacted and deposited in the region of the myosin. The wet-cell allows the addition of water containing ATP and the necessary ions to activate the myosin biomolecules. The near-field optical microscope was shown to able to resolve features of the size of the actin and myosin biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=11144260963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144260963&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:11144260963

SN - 0589-1019

VL - 26 VII

SP - 5250

EP - 5253

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

T2 - Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004

Y2 - 1 September 2004 through 5 September 2004

ER -

Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope

Abstract

All Science Journal Classification (ASJC) codes

Other files and links

Fingerprint

Cite this