Transcription from a TATA-less promoter requires a multisubunit TFIID complex

B. Franklin Pugh, Robert Tjian

Research output: Contribution to journalArticlepeer-review

544 Scopus citations

Abstract

In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).

Original languageEnglish (US)
Pages (from-to)1935-1945
Number of pages11
JournalGenes and Development
Volume5
Issue number11
StatePublished - Nov 1991

All Science Journal Classification (ASJC) codes

  • General Medicine

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