TY - JOUR
T1 - Transcriptional analysis of diverse strains Mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages
AU - Zhu, Xiaochun
AU - Tu, Zheng J.
AU - Coussens, Paul M.
AU - Kapur, Vivek
AU - Janagama, Harish
AU - Naser, Saleh
AU - Sreevatsan, Srinand
N1 - Funding Information:
This work was supported by a USDA-NRI grant (2005-35204-16106). We thank Dr. Josephine Clark-Curtiss for providing pYA1401 plasmid used in these studies.
PY - 2008/10
Y1 - 2008/10
N2 - In this study we analyzed the macrophage-induced gene expression of three diverse genotypes of Mycobacterium avium subsp. paratuberculosis (MAP). Using selective capture of transcribed sequences (SCOTS) on three genotypically diverse MAP isolates from cattle, human, and sheep exposed to primary bovine monocyte derived macrophages for 48 h and 120 h we created and sequenced six cDNA libraries. Sequence annotations revealed that the cattle isolate up-regulated 27 and 241 genes; the human isolate up-regulated 22 and 53 genes, and the sheep isolate up-regulated 35 and 358 genes, at the two time points respectively. Thirteen to thirty-three percent of the genes identified did not have any annotated function. Despite variations in the genes identified, the patterns of expression fell into overlapping cellular functions as inferred by pathway analysis. For example, 10-12% of the genes expressed by all three strains at each time point were associated with cell-wall biosynthesis. All three strains of MAP studied up-regulated genes in pathways that combat oxidative stress, metabolic and nutritional starvation, and cell survival. Taken together, this comparative transcriptional analysis suggests that diverse MAP genotypes respond with similar modus operandi for survival in the host.
AB - In this study we analyzed the macrophage-induced gene expression of three diverse genotypes of Mycobacterium avium subsp. paratuberculosis (MAP). Using selective capture of transcribed sequences (SCOTS) on three genotypically diverse MAP isolates from cattle, human, and sheep exposed to primary bovine monocyte derived macrophages for 48 h and 120 h we created and sequenced six cDNA libraries. Sequence annotations revealed that the cattle isolate up-regulated 27 and 241 genes; the human isolate up-regulated 22 and 53 genes, and the sheep isolate up-regulated 35 and 358 genes, at the two time points respectively. Thirteen to thirty-three percent of the genes identified did not have any annotated function. Despite variations in the genes identified, the patterns of expression fell into overlapping cellular functions as inferred by pathway analysis. For example, 10-12% of the genes expressed by all three strains at each time point were associated with cell-wall biosynthesis. All three strains of MAP studied up-regulated genes in pathways that combat oxidative stress, metabolic and nutritional starvation, and cell survival. Taken together, this comparative transcriptional analysis suggests that diverse MAP genotypes respond with similar modus operandi for survival in the host.
UR - http://www.scopus.com/inward/record.url?scp=54849439916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54849439916&partnerID=8YFLogxK
U2 - 10.1016/j.micinf.2008.07.025
DO - 10.1016/j.micinf.2008.07.025
M3 - Article
C2 - 18692151
AN - SCOPUS:54849439916
SN - 1286-4579
VL - 10
SP - 1274
EP - 1282
JO - Microbes and Infection
JF - Microbes and Infection
IS - 12-13
ER -