Transfection of molecular beacons in microchannels under flow and no-flow conditions

N. Li, P. K. Wong, J. Lin, C. M. Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In this work, transfection of molecular beacons in traditional cell culture dishes and microfluidic cell culture systems with or without flows were comparatively studied by both computational and experimental methods. The comparison between static microfluidic systems and cell culture dishes reveals that the transfection of molecular beacons is controlled by both diffusion and reaction. The study on the microfluidic system with a range of fluid shear stresses and mass transfer rates implies that under high levels of shear stress the disruption of the binding between transfection reagent/molecular beacon complexes and cell membrane is dominant over the enhanced mass transfer.

Original languageEnglish (US)
Title of host publicationMEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages260-263
Number of pages4
DOIs
StatePublished - 2008
Event21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States
Duration: Jan 13 2008Jan 17 2008

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
Country/TerritoryUnited States
CityTucson, AZ
Period1/13/081/17/08

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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