Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

James R. Broach; Jeffrey N. Strathern; James B. Hicks

doi:10.1016/0378-1119(79)90012-X

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

James R. Broach
, Jeffrey N. Strathern
, James B. Hicks

Research output: Contribution to journal › Article › peer-review

744 Scopus citations

Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

Original language	English (US)
Pages (from-to)	121-133
Number of pages	13
Journal	Gene
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(79)90012-X
State	Published - Dec 1979

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0378-1119(79)90012-X

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title = "Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene",

abstract = "We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.",

author = "Broach, \{James R.\} and Strathern, \{Jeffrey N.\} and Hicks, \{James B.\}",

note = "Funding Information: We wish to thank Carolyn McGill and Sajida Ismail for technical help and Louisa Dalessandro for preparation of the manuscript. This work was supported by National Institutes of Health General Medical Sciences grants GM24226 to J.R.B. and GM25624 to J.B.H. and J.N.D.",

year = "1979",

month = dec,

doi = "10.1016/0378-1119(79)90012-X",

language = "English (US)",

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pages = "121--133",

journal = "Gene",

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T1 - Transformation in yeast

T2 - Development of a hybrid cloning vector and isolation of the can1 gene

AU - Broach, James R.

AU - Strathern, Jeffrey N.

AU - Hicks, James B.

N1 - Funding Information: We wish to thank Carolyn McGill and Sajida Ismail for technical help and Louisa Dalessandro for preparation of the manuscript. This work was supported by National Institutes of Health General Medical Sciences grants GM24226 to J.R.B. and GM25624 to J.B.H. and J.N.D.

PY - 1979/12

Y1 - 1979/12

N2 - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

AB - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

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U2 - 10.1016/0378-1119(79)90012-X

DO - 10.1016/0378-1119(79)90012-X

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AN - SCOPUS:0018564346

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VL - 8

SP - 121

EP - 133

JO - Gene

JF - Gene

IS - 1

ER -

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

Abstract

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