Transformation of poplar by agrobacterium tumefaciens

Thomas J. Parsons; Vilas P. Sinkar; Reinhard F. Stettler; Eugene W. Nester; Milton P. Gordon

doi:10.1038/nbt0686-533

Transformation of poplar by agrobacterium tumefaciens

Thomas J. Parsons, Vilas P. Sinkar, Reinhard F. Stettler, Eugene W. Nester, Milton P. Gordon

Forensic Science Program

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Abstract

The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa X deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone autonomous growth, opine production, and Southern blot analysis.

Original language	English (US)
Pages (from-to)	533-536
Number of pages	4
Journal	Bio/Technology
Volume	4
Issue number	6
DOIs	https://doi.org/10.1038/nbt0686-533
State	Published - Jun 1986

All Science Journal Classification (ASJC) codes

Biotechnology

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abstract = "The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa X deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone autonomous growth, opine production, and Southern blot analysis.",

author = "Parsons, {Thomas J.} and Sinkar, {Vilas P.} and Stettler, {Reinhard F.} and Nester, {Eugene W.} and Gordon, {Milton P.}",

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AU - Sinkar, Vilas P.

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AU - Gordon, Milton P.

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AB - The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa X deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone autonomous growth, opine production, and Southern blot analysis.

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Transformation of poplar by agrobacterium tumefaciens

Abstract

All Science Journal Classification (ASJC) codes

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