Translation of insulin granule proteins are regulated by PDI and PABP

Rucha D. Sarwade; Abdul Khalique; Shardul D. Kulkarni; Poonam R. Pandey; Naina Gaikwad; Vasudevan Seshadri

doi:10.1016/j.bbrc.2020.03.106

Translation of insulin granule proteins are regulated by PDI and PABP

Rucha D. Sarwade, Abdul Khalique, Shardul D. Kulkarni, Poonam R. Pandey, Naina Gaikwad, Vasudevan Seshadri

Biochemistry & Molecular Biology

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2 Scopus citations

Abstract

Glucose mediated insulin biosynthesis is tightly regulated and shared between insulin granule proteins such as its processing enzymes, prohormone convertases, PC1/3 and PC2. However, the molecular players involved in the co-ordinated translation remain elusive. The trans-acting factors like PABP (Poly A Binding Protein) and PDI (Protein Disulphide Isomerize) binds to a conserved sequence in the 5′UTR of insulin mRNA and regulates its translation. Here, we demonstrate that 5′UTR of PC1/3 and PC2 also associate with PDI and PABP. We show that a’ and RRM 3–4 domains of PDI and PABP respectively, are necessary for RNA binding activity to the 5′UTRs of insulin and its processing enzymes.

Original language	English (US)
Pages (from-to)	618-625
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	526
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2020.03.106
State	Published - Jun 4 2020

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2020.03.106

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abstract = "Glucose mediated insulin biosynthesis is tightly regulated and shared between insulin granule proteins such as its processing enzymes, prohormone convertases, PC1/3 and PC2. However, the molecular players involved in the co-ordinated translation remain elusive. The trans-acting factors like PABP (Poly A Binding Protein) and PDI (Protein Disulphide Isomerize) binds to a conserved sequence in the 5′UTR of insulin mRNA and regulates its translation. Here, we demonstrate that 5′UTR of PC1/3 and PC2 also associate with PDI and PABP. We show that a{\textquoteright} and RRM 3–4 domains of PDI and PABP respectively, are necessary for RNA binding activity to the 5′UTRs of insulin and its processing enzymes.",

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AU - Sarwade, Rucha D.

AU - Khalique, Abdul

AU - Kulkarni, Shardul D.

AU - Pandey, Poonam R.

AU - Gaikwad, Naina

AU - Seshadri, Vasudevan

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AB - Glucose mediated insulin biosynthesis is tightly regulated and shared between insulin granule proteins such as its processing enzymes, prohormone convertases, PC1/3 and PC2. However, the molecular players involved in the co-ordinated translation remain elusive. The trans-acting factors like PABP (Poly A Binding Protein) and PDI (Protein Disulphide Isomerize) binds to a conserved sequence in the 5′UTR of insulin mRNA and regulates its translation. Here, we demonstrate that 5′UTR of PC1/3 and PC2 also associate with PDI and PABP. We show that a’ and RRM 3–4 domains of PDI and PABP respectively, are necessary for RNA binding activity to the 5′UTRs of insulin and its processing enzymes.

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Translation of insulin granule proteins are regulated by PDI and PABP

Abstract

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