TY - JOUR
T1 - Tumor necrosis factor mediates impaired wound healing in chronic abdominal sepsis
AU - Cooney, Robert
AU - Iocono, Joseph
AU - Maish, George
AU - Smith, J. Stanley
AU - Ehrlich, Paul
PY - 1997/3
Y1 - 1997/3
N2 - Background: The role of systemic tumor necrosis factor (TNF) as a mediator of impaired wound healing in sepsis is unclear. The purpose of this study was to examine the effects of a specific TNF antagonist (TNFbp) on wound healing during chronic abdominal sepsis. Methods: Male Sprague-Dawley rats were divided into four groups: control, control+TNFbp, sepsis, and sepsis+TNFbp. Saline (1.0 mL) or TNFbp (1 mg/kg, 1.0 mL) was injected subcutaneously daily, polyvinylalcohol (PVA) sponge implants were placed in subcutaneous pockets, and sepsis was induced by creation of a chronic, intra- abdominal abscess. Sponge implants were removed on day 5 and examined histologically. Granulation tissue infiltration and quality (connective tissue, cellularity, vascularity) were scored on a scale from 1 to 4 in a blinded fashion. Results: Septic mortality (19 vs. 25%) was not influenced by TNFbp. Granulation tissue penetration and quality were decreased in septic animals. The administration of TNFbp significantly attenuated the effects of sepsis on granulation tissue histology, but not to control levels. Conclusions: These studies provide evidence that TNF contributes to the impaired wound healing observed in this model of chronic abdominal sepsis.
AB - Background: The role of systemic tumor necrosis factor (TNF) as a mediator of impaired wound healing in sepsis is unclear. The purpose of this study was to examine the effects of a specific TNF antagonist (TNFbp) on wound healing during chronic abdominal sepsis. Methods: Male Sprague-Dawley rats were divided into four groups: control, control+TNFbp, sepsis, and sepsis+TNFbp. Saline (1.0 mL) or TNFbp (1 mg/kg, 1.0 mL) was injected subcutaneously daily, polyvinylalcohol (PVA) sponge implants were placed in subcutaneous pockets, and sepsis was induced by creation of a chronic, intra- abdominal abscess. Sponge implants were removed on day 5 and examined histologically. Granulation tissue infiltration and quality (connective tissue, cellularity, vascularity) were scored on a scale from 1 to 4 in a blinded fashion. Results: Septic mortality (19 vs. 25%) was not influenced by TNFbp. Granulation tissue penetration and quality were decreased in septic animals. The administration of TNFbp significantly attenuated the effects of sepsis on granulation tissue histology, but not to control levels. Conclusions: These studies provide evidence that TNF contributes to the impaired wound healing observed in this model of chronic abdominal sepsis.
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U2 - 10.1097/00005373-199703000-00008
DO - 10.1097/00005373-199703000-00008
M3 - Article
C2 - 9095108
AN - SCOPUS:0030909623
SN - 0022-5282
VL - 42
SP - 415
EP - 420
JO - Journal of Trauma - Injury, Infection and Critical Care
JF - Journal of Trauma - Injury, Infection and Critical Care
IS - 3
ER -