Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

R. Steven Sikorski; Lin Wang; Kelli A. Markham; P. T.Ravi Rajagopalan; Stephen J. Benkovic; Amnon Kohen

doi:10.1021/ja031683w

Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

R. Steven Sikorski
, Lin Wang
, Kelli A. Markham
, P. T.Ravi Rajagopalan
, Stephen J. Benkovic
, Amnon Kohen

Chemistry

Research output: Contribution to journal › Article › peer-review

184 Scopus citations

Abstract

H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1° to 2° coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.

Original language	English (US)
Pages (from-to)	4778-4779
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	15
DOIs	https://doi.org/10.1021/ja031683w
State	Published - Apr 21 2004

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja031683w

Cite this

@article{59ff481f532c488d8b40385bbf7aaaac,

title = "Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis",

abstract = "H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1° to 2° coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.",

author = "Sikorski, \{R. Steven\} and Lin Wang and Markham, \{Kelli A.\} and Rajagopalan, \{P. T.Ravi\} and Benkovic, \{Stephen J.\} and Amnon Kohen",

year = "2004",

month = apr,

day = "21",

doi = "10.1021/ja031683w",

language = "English (US)",

volume = "126",

pages = "4778--4779",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "15",

}

TY - JOUR

T1 - Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

AU - Sikorski, R. Steven

AU - Wang, Lin

AU - Markham, Kelli A.

AU - Rajagopalan, P. T.Ravi

AU - Benkovic, Stephen J.

AU - Kohen, Amnon

PY - 2004/4/21

Y1 - 2004/4/21

N2 - H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1° to 2° coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.

AB - H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1° to 2° coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.

UR - https://www.scopus.com/pages/publications/1842830209

UR - https://www.scopus.com/pages/publications/1842830209#tab=citedBy

U2 - 10.1021/ja031683w

DO - 10.1021/ja031683w

M3 - Article

C2 - 15080672

AN - SCOPUS:1842830209

SN - 0002-7863

VL - 126

SP - 4778

EP - 4779

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 15

ER -

Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this