TY - JOUR
T1 - Ubiquitin‐Directed Antibodies Inhibit Neuronal Transporters in Rat Brain Synaptosomes
AU - Meyer, Edwin M.
AU - M. West, Christopher
AU - Stevens, Bruce R.
AU - Chau, Vincent
AU - Nguyen, Minh‐thanh ‐t
AU - Judkins, Jennifer H.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1987/12
Y1 - 1987/12
N2 - Affinity‐purified antibodies specific for ubiquitin were found to inhibit the sodium‐dependent uptake of [3H]choline, r‐[3H]aminobutyric acid ([3H]GABA), [3H]‐glutamate, [3H]norepinephrine, [3H]aspartate, and [3H]scrotonin in rat cerebral cortical synaptosomes at a low concentration (10 μg/ml). These antibodies (termed anti‐Uh) had no effect on the sodium‐independent uptake of these substances or their calcium‐dependent efflux. Synaptosomal [3H]deoxyglucose uptake was not affected in normal Krebs Ringer buffer containing 10 mM glucose, but was inhibited in glucose‐free medium. Other nonneuronal sodium‐dependent transport processes were found to be unaffected by 10 μg/ml anti‐Ub, suggesting that anti‐Ub does not bind indiscriminantly to sodium‐binding sites on sodium‐dependent organic solute transporters. Finally, anti‐Ub inhibited sodium‐dependent [3H]GABA and [3H]glutamate uptake in plasma membrane ghosts, devoid of membrane potential, which were derived from rat cerebral cortical synaptosomes. These results suggest that neuronal transporters or sites proximal to them may be ubiquitinylated on the plasma membrane surface.
AB - Affinity‐purified antibodies specific for ubiquitin were found to inhibit the sodium‐dependent uptake of [3H]choline, r‐[3H]aminobutyric acid ([3H]GABA), [3H]‐glutamate, [3H]norepinephrine, [3H]aspartate, and [3H]scrotonin in rat cerebral cortical synaptosomes at a low concentration (10 μg/ml). These antibodies (termed anti‐Uh) had no effect on the sodium‐independent uptake of these substances or their calcium‐dependent efflux. Synaptosomal [3H]deoxyglucose uptake was not affected in normal Krebs Ringer buffer containing 10 mM glucose, but was inhibited in glucose‐free medium. Other nonneuronal sodium‐dependent transport processes were found to be unaffected by 10 μg/ml anti‐Ub, suggesting that anti‐Ub does not bind indiscriminantly to sodium‐binding sites on sodium‐dependent organic solute transporters. Finally, anti‐Ub inhibited sodium‐dependent [3H]GABA and [3H]glutamate uptake in plasma membrane ghosts, devoid of membrane potential, which were derived from rat cerebral cortical synaptosomes. These results suggest that neuronal transporters or sites proximal to them may be ubiquitinylated on the plasma membrane surface.
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U2 - 10.1111/j.1471-4159.1987.tb02441.x
DO - 10.1111/j.1471-4159.1987.tb02441.x
M3 - Article
C2 - 2890715
AN - SCOPUS:0023617641
SN - 0022-3042
VL - 49
SP - 1815
EP - 1819
JO - Journal of neurochemistry
JF - Journal of neurochemistry
IS - 6
ER -