TY - GEN
T1 - ULTRA LOW ABUNDANCE BIOMOLECULE DETECTION VIA A MICROPORE
AU - Xu, Ruiting
AU - Abune, Lidya
AU - Davis, Brandon
AU - Ouyang, Leixin
AU - Zhang, Ge
AU - Wang, Yong
AU - Zhe, Jiang
N1 - Funding Information:
This research was funded by National Science Foundation, grant number 1905786 and 1911526.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We present a new biomolecule detection method based on nanoparticle counting via a micropore, which can detect ultra-low abundance biomolecules. In this method, aptamer (single stranded oligonucleotide or peptide) modified microparticles with attached complementary sequence (CS)-conjugated silica nanoparticles (SNPs) were used as sensing materials. Target biomolecules (e.g. adenosine) induce conformational dissociation of complementary sequence - aptamer complex and release SNPs from the microparticles. A resistive pulse sensor with a micropore is then used to detect the released nanoparticles. From the number of the released nanoparticles, biomolecule concentration can be determined digitally.
AB - We present a new biomolecule detection method based on nanoparticle counting via a micropore, which can detect ultra-low abundance biomolecules. In this method, aptamer (single stranded oligonucleotide or peptide) modified microparticles with attached complementary sequence (CS)-conjugated silica nanoparticles (SNPs) were used as sensing materials. Target biomolecules (e.g. adenosine) induce conformational dissociation of complementary sequence - aptamer complex and release SNPs from the microparticles. A resistive pulse sensor with a micropore is then used to detect the released nanoparticles. From the number of the released nanoparticles, biomolecule concentration can be determined digitally.
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M3 - Conference contribution
AN - SCOPUS:85136944025
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1623
EP - 1624
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -