TY - JOUR
T1 - Ultrafiltration behavior of bacterial polysaccharides used in vaccines
AU - Hadidi, Mahsa
AU - Buckley, John J.
AU - Zydney, Andrew L.
N1 - Funding Information:
The authors would like to acknowledge Pfizer Inc. for their financial support and for providing the purified polysaccharides. EMD Millipore donated the Ultracel TM and Biomax TM membranes used in these studies.
PY - 2015/9/5
Y1 - 2015/9/5
N2 - Ultrafiltration is widely used for the purification of polysaccharide-based vaccines against pneumococci and meningococci, but there is currently no fundamental understanding of the factors controlling the ultrafiltration behavior of these polysaccharides. Experiments were performed using purified pneumococcus polysaccharides (serotypes Pn3, Pn9V, and Pn14) provided by Pfizer. Ultrafiltration data were obtained in a stirred cell using Ultracel™ composite regenerated cellulose and Biomax™ polyethersulfone membranes with different nominal molecular weight cutoffs. Polysaccharide transmission was a strong function of filtrate flux, with data at low bulk concentrations in good agreement with the classical concentration polarization model. Polysaccharide fouling became significant at high filtrate flux during ultrafiltration of more concentrated solutions, consistent with the presence of a critical value for the wall concentration. Polysaccharide transmission was in good agreement with available hydrodynamic models accounting for the presence of a pore size distribution, with the effective size of the polysaccharide determined by dynamic light scattering or size exclusion chromatography. These results provide important insights into the factors controlling the ultrafiltration behavior of bacterial polysaccharides of interest in bioprocessing applications.
AB - Ultrafiltration is widely used for the purification of polysaccharide-based vaccines against pneumococci and meningococci, but there is currently no fundamental understanding of the factors controlling the ultrafiltration behavior of these polysaccharides. Experiments were performed using purified pneumococcus polysaccharides (serotypes Pn3, Pn9V, and Pn14) provided by Pfizer. Ultrafiltration data were obtained in a stirred cell using Ultracel™ composite regenerated cellulose and Biomax™ polyethersulfone membranes with different nominal molecular weight cutoffs. Polysaccharide transmission was a strong function of filtrate flux, with data at low bulk concentrations in good agreement with the classical concentration polarization model. Polysaccharide fouling became significant at high filtrate flux during ultrafiltration of more concentrated solutions, consistent with the presence of a critical value for the wall concentration. Polysaccharide transmission was in good agreement with available hydrodynamic models accounting for the presence of a pore size distribution, with the effective size of the polysaccharide determined by dynamic light scattering or size exclusion chromatography. These results provide important insights into the factors controlling the ultrafiltration behavior of bacterial polysaccharides of interest in bioprocessing applications.
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U2 - 10.1016/j.memsci.2015.04.047
DO - 10.1016/j.memsci.2015.04.047
M3 - Article
AN - SCOPUS:84930226559
SN - 0376-7388
VL - 490
SP - 294
EP - 300
JO - Journal of Membrane Science
JF - Journal of Membrane Science
ER -
