TY - JOUR
T1 - Ultrafiltration of PEGylated proteins
T2 - Fouling and concentration polarization effects
AU - Kwon, Boksoon
AU - Molek, Jessica
AU - Zydney, Andrew L.
N1 - Funding Information:
This work was supported in part by a Korea Research Foundation Grant by the Korean Government (MOEHRD) (KRF-2006-352-D00038). The authors would also like to acknowledge Millipore Corp. for donation of the Ultracel ® and Biomax ® membranes and Pfizer Corp. for donation of the 12 kDa PEGylated BSA.
PY - 2008/7/1
Y1 - 2008/7/1
N2 - One of the critical factors governing the application of membrane technology for the commercial production of PEGylated proteins is membrane fouling. The objective of this study was to examine the effects of PEGylation on the filtrate flux and fouling characteristics during ultrafiltration of a model PEGylated protein using both fully and partially retentive regenerated cellulose and polyethersulfone membranes. The physical characteristics of the native and PEGylated proteins were evaluated by size exclusion chromatography, dynamic light scattering, capillary electrophoresis, and reverse-phase chromatography. The filtration behavior with the cellulose membranes was dominated by concentration polarization effects, with the lower flux for the PEGylated proteins associated with the smaller bulk mass transfer coefficient. Significant fouling was observed with the polyethersulfone membranes. Greater fouling was seen with the PEGylated protein due to its increased size, greater hydrophobicity, and lower electrostatic interactions, all of which are directly associated with the attached poly(ethylene glycol) chains. These results provide important insights into the ultrafiltration characteristics of PEGylated proteins.
AB - One of the critical factors governing the application of membrane technology for the commercial production of PEGylated proteins is membrane fouling. The objective of this study was to examine the effects of PEGylation on the filtrate flux and fouling characteristics during ultrafiltration of a model PEGylated protein using both fully and partially retentive regenerated cellulose and polyethersulfone membranes. The physical characteristics of the native and PEGylated proteins were evaluated by size exclusion chromatography, dynamic light scattering, capillary electrophoresis, and reverse-phase chromatography. The filtration behavior with the cellulose membranes was dominated by concentration polarization effects, with the lower flux for the PEGylated proteins associated with the smaller bulk mass transfer coefficient. Significant fouling was observed with the polyethersulfone membranes. Greater fouling was seen with the PEGylated protein due to its increased size, greater hydrophobicity, and lower electrostatic interactions, all of which are directly associated with the attached poly(ethylene glycol) chains. These results provide important insights into the ultrafiltration characteristics of PEGylated proteins.
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U2 - 10.1016/j.memsci.2008.03.035
DO - 10.1016/j.memsci.2008.03.035
M3 - Article
AN - SCOPUS:44249102502
SN - 0376-7388
VL - 319
SP - 206
EP - 213
JO - Journal of Membrane Science
JF - Journal of Membrane Science
IS - 1-2
ER -