Ultrasound-modulated optical microscopy for ex vivo imaging of scattering biological tissue

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Ultrasound-modulated optical microscopy (UOM) based on a long-cavity confocal Fabry-Perot interferometer (CFPI) [J. Biomed. Opt. 13(5), 0504046, (2008)] is used for real time detection of multiply scattered light modulated by high frequency (30 MHz) ultrasound pulses propagating in an optically strongly scattering medium. In this article, we use this microscope to study the dependence of ultrasound-modulated optical signals on the optical absorption of objects embedded about 3 mm deep in tissue mimicking phantoms. These results demonstrate that the dependence is nearly linear. Most importantly, we imaged blood vasculature and melanin in highly scattering tissue samples from a mouse and a rat. Thus UOM can be used to study the morphology of blood vasculature and blood-associated functional parameters, such as oxygen saturation.

Original languageEnglish (US)
Article number71772N
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2009
EventPhotons Plus Ultrasound: Imaging and Sensing 2009 - San Jose, CA, United States
Duration: Jan 25 2009Jan 28 2009

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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